Abstract
The nuclear polyhedrosis virus of Autographa californica has been titrated in Spodoptera frugiperda cells by the plaque method, using a solid overlay which does not require either the use of modified culture medium or expensive purified agarose or the addition of culture medium as a liquid layer above the solid agarose. This assay is more sensitive than that using a viscous methyl cellulose overlay but less sensitive than the end-point dilution technique. Neither Trichoplusia ni nor Bombyx mori cells were satisfactory as indicators for the assay as described, since they failed to form a stable monolayer. Manduca sexta cells could be utilized for assay of A. californica nuclear polyhedrosis virus, but the sensitivity was lower than with S. frugiperda cells.
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Selected References
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- Eide P. E., Caldwell J. M., Marks E. P. Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L). In Vitro. 1975 Nov-Dec;11(6):395–399. doi: 10.1007/BF02616376. [DOI] [PubMed] [Google Scholar]
- Grace T. D. Establishment of a line of cells from the silkworm Bombyx mori. Nature. 1967 Nov 11;216(5115):613–613. doi: 10.1038/216613a0. [DOI] [PubMed] [Google Scholar]
- Hink W. F. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature. 1970 May 2;226(5244):466–467. doi: 10.1038/226466b0. [DOI] [PubMed] [Google Scholar]
- Potter K. N., Faulkner P., MacKinnon E. A. Strain selection during serial passage of Trichoplusia in nuclear polyhedrosis virus. J Virol. 1976 Jun;18(3):1040–1050. doi: 10.1128/jvi.18.3.1040-1050.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 1977 Apr;13(4):213–217. doi: 10.1007/BF02615077. [DOI] [PubMed] [Google Scholar]