Abstract
Bacterial counts obtained on hydrophobic grid-membrane filters were comparable to conventional plate counts for Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus in homogenates from a range of foods. The wide numerical operating range of the hydrophobic grid-membrane filters allowed sequential diluting to be reduced or even eliminated, making them attractive as components in automated systems of analysis. Food debris could be rinsed completely from the unincubated hydrophobic grid-membrane filter surface without affecting the subsequent count, thus eliminating the possibility of counting food particles, a common source of error in electronic counting systems.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Sharpe A. N., Jackson A. K. Stomaching: a new concept in bacteriological sample preparation. Appl Microbiol. 1972 Aug;24(2):175–178. doi: 10.1128/am.24.2.175-178.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe A. N. Machine for printing hydrophobic grids on membrane filters. Appl Microbiol. 1975 Jul;30(1):110–112. doi: 10.1128/am.30.1.110-112.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe A. N., Michaud G. L. Enumeration of high numbers of bacteria using hydrophobic grid-membrane filters. Appl Microbiol. 1975 Oct;30(4):519–524. doi: 10.1128/am.30.4.519-524.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe A. N., Michaud G. L. Hydrophobic grid-membrane filters: new approach to microbiological enumeration. Appl Microbiol. 1974 Aug;28(2):223–225. doi: 10.1128/am.28.2.223-225.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]