Abstract
A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.
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Selected References
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- ANGUS T. A. Extraction, purification, and properties of Bacillus sotto toxin. Can J Microbiol. 1956 Jun;2(4):416–426. doi: 10.1139/m56-049. [DOI] [PubMed] [Google Scholar]
- Delafield F. P., Somerville H. J., Rittenberg S. C. Immunological homology between crystal and spore protein of Bacillus thuringiensis. J Bacteriol. 1968 Sep;96(3):713–720. doi: 10.1128/jb.96.3.713-720.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodman N. S., Gottfried R. J., Rogoff M. H. Biphasic system for separation of spores and crystals of Bacillus thuringiensis. J Bacteriol. 1967 Aug;94(2):485–485. doi: 10.1128/jb.94.2.485-.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe E. S., Nickerson K. W., Bulla L. A., Jr, Aronson J. N. Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents. Appl Microbiol. 1975 Dec;30(6):1052–1053. doi: 10.1128/am.30.6.1052-1053.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tamir H., Gilvarg C. Density gradient centrifugation for the separation of sporulating forms of bacteria. J Biol Chem. 1966 Mar 10;241(5):1085–1090. [PubMed] [Google Scholar]
- Wise J., Swanson A., Halvorson H. O. Dipicolinic acid-less mutants of Bacillus cereus. J Bacteriol. 1967 Dec;94(6):2075–2076. doi: 10.1128/jb.94.6.2075-2076.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]