Abstract
Protohemin and covalently bound hemin were determined in eight aerobic bacterial strains. A good correlation between protohemin content and luminol reactivity was found. The ratio of luminol reaction to protohemin for the eight investigated strains was essentially identical to that of pure protohemin, 0.7 X 10(16) mV/mol. Covalently bound hemin contributed to the chemiluminescence to a minor extent only (0.7 X 10(14) mV/mol, in accordance with earlier observations of the lower reactivity of cytochrome c and related compounds. A difference in reaction kinetics of the luminol reaction with covalently bound hemin (slower reaction than protohemin) and protohemin was observed in vivo as well as in vitro. The phenomenon could be used to differentiate between strains with different hemin composition.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cox R., Charles H. P. Porphyrin-accumulating mutants of Escherichia coli. J Bacteriol. 1973 Jan;113(1):122–132. doi: 10.1128/jb.113.1.122-132.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ewetz L., Strangert K. A simple method for detection of bacteriuria with an automated chemiluminescence technique. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):375–381. doi: 10.1111/j.1699-0463.1974.tb02340.x. [DOI] [PubMed] [Google Scholar]
- Ewetz L., Thore A. Factors affecting the specificity of the luminol reaction with hematin compounds. Anal Biochem. 1976 Apr;71(2):564–570. doi: 10.1016/s0003-2697(76)80025-5. [DOI] [PubMed] [Google Scholar]
- Lundin A., Thore A. Analytical information obtainable by evaluation of the time course of firefly bioluminescence in the assay of ATP. Anal Biochem. 1975 May 26;66(1):47–63. doi: 10.1016/0003-2697(75)90723-x. [DOI] [PubMed] [Google Scholar]
- Michelson A. M. Studies in bioluminescence. 10. Chemical models of enzymic oxidations. Biochimie. 1973;55(4):465–479. doi: 10.1016/s0300-9084(73)80213-5. [DOI] [PubMed] [Google Scholar]
- NEUFELD H. A., CONKLIN C. J., TOWNER R. D. CHEMILUMINESCENCE OF LUMINOL IN THE PRESENCE OF HEMATIN COMPOUNDS. Anal Biochem. 1965 Aug;12:303–309. doi: 10.1016/0003-2697(65)90095-3. [DOI] [PubMed] [Google Scholar]
- Sotnikov G. G. Detection of iron-porphyrin proteins with a biochemiluminescent method in search of extraterrestrial life. Life Sci Space Res. 1970;8:90–98. [PubMed] [Google Scholar]