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. 1979 Mar;37(3):454–458. doi: 10.1128/aem.37.3.454-458.1979

Nitrogen Fixation by Thermophilic Blue-Green Algae (Cyanobacteria): Temperature Characteristics and Potential Use in Biophotolysis

Kazuhisa Miyamoto 1,†, Patrick C Hallenbeck 1, John R Benemann 1
PMCID: PMC243238  PMID: 16345353

Abstract

Thermophilic, nitrogen-fixing, blue-green algae (cyanobacteria) were investigated for use in biophotolysis. Three strains of Mastigocladus laminosus were tested and were found to be equally effective in biophotolysis as judged by nitrogenase activity. The alga, M. laminosus NZ-86-m, which was chosen for further study, grew well in the temperature range from 35 to 50°C, with optimum growth at 45°C, at which temperature acetylene reduction activity was also greatest. The maximum tolerable temperature was 55°C. Acetylene reduction activity was saturated at a light intensity of 1 × 104 ergs cm−2 s−1. Atmospheric oxygen tension was found to be slightly inhibitory to acetylene reduction of both slowly growing and exponentially growing cultures. Nonsterile continuous cultures, which were conducted to test problems of culture maintenance, could be operated for 2 months without any significant decrease in nitrogenase activity or contamination by other algae. Nitrogen-starved cultures of M. laminosus NZ-86-m produced hydrogen at comparable rates to Anabaena cylindrica. The conversion efficiency of light to hydrogen energy at maximum rates of hydrogen production was 2.7%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benemann J. R., Weare N. M. Hydrogen Evolution by Nitrogen-Fixing Anabaena cylindrica Cultures. Science. 1974 Apr 12;184(4133):174–175. doi: 10.1126/science.184.4133.174. [DOI] [PubMed] [Google Scholar]
  3. Castenholz R. W. Thermophilic blue-green algae and the thermal environment. Bacteriol Rev. 1969 Dec;33(4):476–504. doi: 10.1128/br.33.4.476-504.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jeffries T. W., Timourian H., Ward R. L. Hydrogen production by Anabaena cylindrica: effects of varying ammonium and ferric ions, pH, and light. Appl Environ Microbiol. 1978 Apr;35(4):704–710. doi: 10.1128/aem.35.4.704-710.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schneider K. C., Bradbeer C., Singh R. N., Wang L. C., Wilson P. W., Burris R. H. NITROGEN FIXATION BY CELL-FREE PREPARATIONS FROM MICROORGANISMS. Proc Natl Acad Sci U S A. 1960 May;46(5):726–733. doi: 10.1073/pnas.46.5.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Weare N. M., Benemann J. R. Nitrogen fixation by Anabaena cylindrica. II. Nitrogenase activity during induction and aging of batch cultures. Arch Mikrobiol. 1973 Oct 19;93(2):101–112. [PubMed] [Google Scholar]
  7. Weissman J. C., Benemann J. R. Hydrogen production by nitrogen-starved cultures of Anabaena cylindrica. Appl Environ Microbiol. 1977 Jan;33(1):123–131. doi: 10.1128/aem.33.1.123-131.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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