Abstract
The procaryotic endogenous storage material poly-β-hydroxybutyrate (PHB) can be induced to accumulate in the estuarine detrital microbiota under conditions which suggest unbalanced growth, such as limitation of a critical factor(s) in the presence of carbon and energy sources. Changes in PHB-to-lipid phosphate ratios detected in field samples can be mimicked in the laboratory with common estuarine stresses. Acute anoxia or low pH induces conditions of no growth with depression of both the synthesis and catabolism of PHB without change in the lipid phosphate. Balanced growth induced by nutrients increases the lipid phosphate, depresses PHB synthesis, and stimulates PHB catabolism, resulting in a low ratio of PHB to lipid phosphate. Unbalanced growth induced to a small extent by high salinity or much more readily by dark upland runoff water results in rapid accumulation of PHB and slowing of PHB catabolism with little change in lipid phospate. Unbalanced growth conditions result in high PHB-to-lipid phosphate ratios in the detrital microbiota.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Bobbie R. J., Morrison S. J., White D. C. Effects of substrate biodegradability on the mass and activity of the associated estuarine microbiota. Appl Environ Microbiol. 1978 Jan;35(1):179–184. doi: 10.1128/aem.35.1.179-184.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawes E. A., Senior P. J. The role and regulation of energy reserve polymers in micro-organisms. Adv Microb Physiol. 1973;10:135–266. doi: 10.1016/s0065-2911(08)60088-0. [DOI] [PubMed] [Google Scholar]
- Herron J. S., King J. D., White D. C. Recovery of Poly-beta-Hydroxybutyrate from Estuarine Microflora. Appl Environ Microbiol. 1978 Feb;35(2):251–257. doi: 10.1128/aem.35.2.251-257.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
- King J. D., White D. C., Taylor C. W. Use of lipid composition and metabolism to examine structure and activity of estuarine detrital microflora. Appl Environ Microbiol. 1977 May;33(5):1177–1183. doi: 10.1128/aem.33.5.1177-1183.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morman M. R., White D. C. Phospholipid metabolism during penicillinase production in Bacillus licheniformis. J Bacteriol. 1970 Oct;104(1):247–253. doi: 10.1128/jb.104.1.247-253.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Short S. A., White D. C., Aleem M. I. Phospholipid metabolism in Ferrobacillus ferrooxidans. J Bacteriol. 1969 Jul;99(1):142–150. doi: 10.1128/jb.99.1.142-150.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White D. C., Tucker A. N. Phospholipid metabolism during bacterial growth. J Lipid Res. 1969 Mar;10(2):220–233. [PubMed] [Google Scholar]
- White D. C., Tucker A. N. Phospholipid metabolism during changes in the proportions of membrane-bound respiratory pigments in Haemophilus parainfluenzae. J Bacteriol. 1969 Jan;97(1):199–209. doi: 10.1128/jb.97.1.199-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]