Abstract
An aerobic endospore-forming bacterium, tentatively identified as a strain (JJ-lb) of Bacillus macerans, was isolated by enrichment on 4-hydroxybenzoate (4HBA), using as an inoculum soil taken from a 50 degrees C Iadho hot spring. Enzymatic analyses of cells grown on succinate and 4HBA indicated that strain JJ-1b degrades 4HBA by way of the novel protocatechuate (PCA) 2,3-dioxygenase pathway. Purification of the PCA 2,3-dioxygenase by affinity chromatography allowed the first observation of the immediate ring fission product of PCA, namely, 5-carboxy-2-hydroxymuconic semialdehyde (CHMS; labmda max at pH 7.0 = 348 nm). An affinity column fraction was obtained that decarboxylated CHMS to 2-hydroxymuconic semialdehyde (HMS; lambdamax at pH 7.0 = 375 nm). Thus, conversion of PCA to HMS is accomplished in two steps, 2,3-fission of the PCA ring followed by enzymatic decarboxylation of the ring fission product, forming HMS.
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- Bayly R. C., Dagley S., Gibson D. T. The metabolism of cresols by species of Pseudomonas. Biochem J. 1966 Nov;101(2):293–301. doi: 10.1042/bj1010293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collinsworth W. L., Chapman P. J., Dagley S. Stereospecific enzymes in the degradation of aromatic compounds by pseudomonas putida. J Bacteriol. 1973 Feb;113(2):922–931. doi: 10.1128/jb.113.2.922-931.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford R. L. Degradation of 3-hydroxybenzoate by bacteria of the genus Bacillus. Appl Microbiol. 1975 Sep;30(3):439–444. doi: 10.1128/am.30.3.439-444.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford R. L. Novel pathway for degradation of protocatechuic acid in Bacillus species. J Bacteriol. 1975 Feb;121(2):531–536. doi: 10.1128/jb.121.2.531-536.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford R. L. Pathways of 4-hydroxybenzoate degradation among species of Bacillus. J Bacteriol. 1976 Jul;127(1):204–210. doi: 10.1128/jb.127.1.204-210.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dagley S. Catabolism of aromatic compounds by micro-organisms. Adv Microb Physiol. 1971;6(0):1–46. doi: 10.1016/s0065-2911(08)60066-1. [DOI] [PubMed] [Google Scholar]
- DeFrank J. J., Ribbons D. W. p-Cymene pathway in Pseudomonas putida: ring cleavage of 2,3-dihydroxy-p-cumate and subsequent reactions. J Bacteriol. 1977 Mar;129(3):1365–1374. doi: 10.1128/jb.129.3.1365-1374.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeFrank J. J., Ribbons D. W. p-cymene pathway in Pseudomonas putida: initial reactions. J Bacteriol. 1977 Mar;129(3):1356–1364. doi: 10.1128/jb.129.3.1356-1364.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jamaluddin M. P. Purification and properties of homoprotocatechuate 2,3-dioxygenase from Bacillus stearothermophilus. J Bacteriol. 1977 Feb;129(2):690–697. doi: 10.1128/jb.129.2.690-697.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Nozaki M., Kotani S., Ono K., Seno S. Metapyrocatechase. 3. Substrate specificity and mode of ring fission. Biochim Biophys Acta. 1970 Nov 11;220(2):213–223. doi: 10.1016/0005-2744(70)90007-0. [DOI] [PubMed] [Google Scholar]
- Ornston L. N., Stanier R. Y. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. J Biol Chem. 1966 Aug 25;241(16):3776–3786. [PubMed] [Google Scholar]
- Ribbons D. W., Senior P. J. 2,3-dihydroxybenzoate 3,4-oxygenase from Pseudomonas fluorescens--oxidation of a substrate analog. Arch Biochem Biophys. 1970 Jun;138(2):557–565. doi: 10.1016/0003-9861(70)90381-4. [DOI] [PubMed] [Google Scholar]
- Sala-Trepat J. M., Evans W. C. The meta cleavage of catechol by Azotobacter species. 4-Oxalocrotonate pathway. Eur J Biochem. 1971 Jun 11;20(3):400–413. doi: 10.1111/j.1432-1033.1971.tb01406.x. [DOI] [PubMed] [Google Scholar]