Abstract
To determine possible pathogenesis of Vibrio parahaemolyticus-host-organ system interactions, studies of invasiveness were made by a direct fluorescent-antibody method. Broth cultures of live cells isolated from seafish or symptomatic humans were inoculated separately into ligated ileal loops of young New Zealand white rabbits. After suitable incubation, rabbits were sacrificed, and ileal loops and tissue specimens were aseptically removed. Ileal loops were prepared and stained with specific fluorescein-tagged antibody, and organ specimens were cultured for isolation of the inoculated Vibrio strain. All strains tested penetrated into the lamina propria of the ileum and were isolated from the cultured tissue specimens, indicating that the organism is capable of more than a superficial colonization of the gut. The presence of Vibrio in cultured tissue specimens suggests invasion of deeper tissue by either the lymphatic or the circulatory system.
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- Brown D. F., Spaulding P. L., Twedt R. M. Enteropathogenicity of Vibrio parahaemolyticus in the ligated rabbit ileum. Appl Environ Microbiol. 1977 Jan;33(1):10–14. doi: 10.1128/aem.33.1.10-14.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Calia F. M., Johnson D. E. Bacteremia in suckling rabbits after oral challenge with Vibrio parahaemolyticus. Infect Immun. 1975 Jun;11(6):1222–1225. doi: 10.1128/iai.11.6.1222-1225.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carruthers M. M. Cytotoxicity of Vibrio parahaemolyticus in HeLa cell culture. J Infect Dis. 1975 Nov;132(5):555–560. doi: 10.1093/infdis/132.5.555. [DOI] [PubMed] [Google Scholar]
- DEDMON R. E., HOLMES A. W., DEINHARDT F. PREPARATION OF FLUORESCEIN ISOTHIOCYANATE-LABELED GAMMA-GLOBULIN BY DIALYSIS, GEL FILTRATION, AND IONEXCHANGE CHROMATOGRAPHY IN COMBINATION. J Bacteriol. 1965 Mar;89:734–739. doi: 10.1128/jb.89.3.734-739.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Shimizu M., Takeda Y., Miwatani T. Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus. Infect Immun. 1976 Oct;14(4):1028–1033. doi: 10.1128/iai.14.4.1028-1033.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakazaki R., Tamura K., Kato T., Obara Y., Yamai S. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenicity. Jpn J Med Sci Biol. 1968 Oct;21(5):325–331. doi: 10.7883/yoken1952.21.325. [DOI] [PubMed] [Google Scholar]
- Sanyal S. C., Sil J., Sakazaki R. Laboratory infection by Vibrio parahaemolyticus. J Med Microbiol. 1973 Feb;6(1):121–122. doi: 10.1099/00222615-6-1-121. [DOI] [PubMed] [Google Scholar]