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. 1979 Apr;37(4):670–675. doi: 10.1128/aem.37.4.670-675.1979

Effect of inorganic sulfide on the growth and metabolism of Methanosarcina barkeri strain DM.

D O Mountfort, R A Asher
PMCID: PMC243279  PMID: 572199

Abstract

Minimal growth of Methanosarcina barkeri strain DM occurred when sulfide was omitted fromthe growth medium, and addition of either sodium sulfate or coenzyme M to sulfide-depleted media failed to restore growth. Optimal growth occurred in the presence of 1.25 mM added sulfide, giving a molar growth yield (YCH4) of 4.4 mg (dry weight) of cells per mmol of CH4 produced. Increasing sulfide to 12.5 mM led to decrease in YCH4 (1.9 mg [dry weight]/mmol of CH4), in the specific growth rate and in be intracellular levels of adenosine triphosphate. However, the specific rate of methane production increased. The data suggested that at elevated sulfide levels (12.5 mM) the decrease in YCH4 might be a result of an increase in the relative energy needed for maintnenace and of uncoupling of growth from energy production.

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Selected References

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  1. Abram J. W., Nedwell D. B. Hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment. Arch Microbiol. 1978 Apr 27;117(1):93–97. doi: 10.1007/BF00689357. [DOI] [PubMed] [Google Scholar]
  2. Abram J. W., Nedwell D. B. Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Arch Microbiol. 1978 Apr 27;117(1):89–92. doi: 10.1007/BF00689356. [DOI] [PubMed] [Google Scholar]
  3. Blaylock B. A., Stadtman T. C. Methane biosynthesis by Methanosarcina barkeri. Properties of the soluble enzyme system. Arch Biochem Biophys. 1966 Sep 26;116(1):138–152. doi: 10.1016/0003-9861(66)90022-1. [DOI] [PubMed] [Google Scholar]
  4. Bryant M. P., Campbell L. L., Reddy C. A., Crabill M. R. Growth of desulfovibrio in lactate or ethanol media low in sulfate in association with H2-utilizing methanogenic bacteria. Appl Environ Microbiol. 1977 May;33(5):1162–1169. doi: 10.1128/aem.33.5.1162-1169.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cappenberg T. E. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. I. Field observations. Antonie Van Leeuwenhoek. 1974;40(2):285–295. doi: 10.1007/BF00394387. [DOI] [PubMed] [Google Scholar]
  6. Ferry J. G., Wolfe R. S. Nutritional and biochemical characterization of Methanospirillum hungatii. Appl Environ Microbiol. 1977 Oct;34(4):371–376. doi: 10.1128/aem.34.4.371-376.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Forsberg C. W., Lam K. Use of adenosine 5'-triphosphate as an indicator of the microbiota biomass in rumen contents. Appl Environ Microbiol. 1977 Mar;33(3):528–537. doi: 10.1128/aem.33.3.528-537.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gunsalus R. P., Wolfe R. S. ATP activation and properties of the methyl coenzyme M reductase system in Methanobacterium thermoautotrophicum. J Bacteriol. 1978 Sep;135(3):851–857. doi: 10.1128/jb.135.3.851-857.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gunsalus R. P., Wolfe R. S. Stimulation of CO2 reduction to methane by methylcoenzyme M in extracts Methanobacterium. Biochem Biophys Res Commun. 1977 Jun 6;76(3):790–795. doi: 10.1016/0006-291x(77)91570-4. [DOI] [PubMed] [Google Scholar]
  10. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Khan A. W., Trottier T. M. Effect of sulfur-containing compounds on anaerobic degradation of cellulose to methane by mixed cultures obtained from sewage sludge. Appl Environ Microbiol. 1978 Jun;35(6):1027–1034. doi: 10.1128/aem.35.6.1027-1034.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mah R. A., Smith M. R., Baresi L. Studies on an acetate-fermenting strain of Methanosarcina. Appl Environ Microbiol. 1978 Jun;35(6):1174–1184. doi: 10.1128/aem.35.6.1174-1184.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mountfort D. O., Asher R. A. Changes in proportions of acetate and carbon dioxide used as methane precursors during the anaerobic digestion of bovine waste. Appl Environ Microbiol. 1978 Apr;35(4):648–654. doi: 10.1128/aem.35.4.648-654.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. POSTGATE J. R. Versatile medium for the enumeration of sulfate-reducing bacteria. Appl Microbiol. 1963 May;11:265–267. doi: 10.1128/am.11.3.265-267.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pirt S. J. The maintenance energy of bacteria in growing cultures. Proc R Soc Lond B Biol Sci. 1965 Oct 12;163(991):224–231. doi: 10.1098/rspb.1965.0069. [DOI] [PubMed] [Google Scholar]
  16. Roberton A. M., Wolfe R. S. Adenosine triphosphate pools in Methanobacterium. J Bacteriol. 1970 Apr;102(1):43–51. doi: 10.1128/jb.102.1.43-51.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stouthamer A. H. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie Van Leeuwenhoek. 1973;39(3):545–565. doi: 10.1007/BF02578899. [DOI] [PubMed] [Google Scholar]
  18. Stouthamer A. H., Bettenhaussen C. Influence of hydrogen acceptors on growth and energy production of Proteus mirabilis. Antonie Van Leeuwenhoek. 1972;38(1):81–90. doi: 10.1007/BF02328079. [DOI] [PubMed] [Google Scholar]
  19. Stouthamer A. H., Bettenhaussen C. Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. A reevaluation of the method for the determination of ATP production by measuring molar growth yields. Biochim Biophys Acta. 1973 Feb 12;301(1):53–70. doi: 10.1016/0304-4173(73)90012-8. [DOI] [PubMed] [Google Scholar]
  20. TRUEPER H. G., SCHLEGEL H. G. SULPHUR METABOLISM IN THIORHODACEAE. I. QUANTITATIVE MEASUREMENTS ON GROWING CELLS OF CHROMATIUM OKENII. Antonie Van Leeuwenhoek. 1964;30:225–238. doi: 10.1007/BF02046728. [DOI] [PubMed] [Google Scholar]
  21. Taylor C. D., Wolfe R. S. Structure and methylation of coenzyme M(HSCH2CH2SO3). J Biol Chem. 1974 Aug 10;249(15):4879–4885. [PubMed] [Google Scholar]
  22. Thauer R. K., Jungermann K., Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977 Mar;41(1):100–180. doi: 10.1128/br.41.1.100-180.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WOLIN E. A., WOLIN M. J., WOLFE R. S. FORMATION OF METHANE BY BACTERIAL EXTRACTS. J Biol Chem. 1963 Aug;238:2882–2886. [PubMed] [Google Scholar]
  24. Wellinger A., Wuhrmann K. Influence of sulfide compounds on the metabolism of Methanobacterium strain AZ. Arch Microbiol. 1977 Oct 24;115(1):13–17. doi: 10.1007/BF00427839. [DOI] [PubMed] [Google Scholar]
  25. Winfrey M. R., Zeikus J. G. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl Environ Microbiol. 1977 Feb;33(2):275–281. doi: 10.1128/aem.33.2.275-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zeikus J. G. The biology of methanogenic bacteria. Bacteriol Rev. 1977 Jun;41(2):514–541. doi: 10.1128/br.41.2.514-541.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zhilina T. N. Biotipy metanosartsiny. Mikrobiologiia. 1976 May-Jun;45:481–489. [PubMed] [Google Scholar]
  28. van Uden N. Kinetics of nutrient-limited growth. Annu Rev Microbiol. 1969;23:473–486. doi: 10.1146/annurev.mi.23.100169.002353. [DOI] [PubMed] [Google Scholar]

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