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. 1979 Apr;37(4):725–728. doi: 10.1128/aem.37.4.725-728.1979

Secondary selective enrichment of salmonellae from naturally contaminated specimens by using a selective motility system.

T I Smeltzer, F Duncalfe
PMCID: PMC243288  PMID: 453839

Abstract

A selective motility medium was used as a secondary selective enrichment medium to examine specimens naturally contaminated with salmonellae. The medium, incubated at 37 degrees C, was inoculated from either selenite brilliant green sulfa enrichment broth or Müller-Kauffman tetrathionate broth, both of which had been incubated at 42 degrees C. The use of the selective motility medium resulted in an increase in the number of positive specimens from 65 and 74% to 80 and 82%, when inoculated at 24 and 48 h, respectively, from tetrathionate broth. Tetrathionate broth, when used singly, was significantly better than selenite brilliant green sulfa broth, which detected 55% of positive specimens at both 24 and 48 h. The use of the selective motility medium of Harper and Shortridge (J. Hyg. 67: 181--186, 1969) for the further examination of specimens culturally negative on primary selective enrichment is advocated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlson V. L., Snoeyenbos G. H., McKie B. A., Smyser C. F. A comparison of incubation time and temperature for the isolation of salmonella. Avian Dis. 1967 May;11(2):217–225. [PubMed] [Google Scholar]
  2. Edel W., Kampelmacher E. H. Comparative studies on Salmonella isolations from feeds in ten laboratories. Bull World Health Organ. 1974;50(5):421–426. [PMC free article] [PubMed] [Google Scholar]
  3. Edel W., Kampelmacher E. H. Comparative studies on the isolation of "sublethally injured" salmonellae in nine European laboratories. Bull World Health Organ. 1973;48(2):167–174. [PMC free article] [PubMed] [Google Scholar]
  4. Greenfield J., Bankier J. C. Isolation of Salmonella and Arizona using enrichment media incubated at 35 and 43 c. Avian Dis. 1969 Nov;13(4):864–871. [PubMed] [Google Scholar]
  5. Harper J., Shortridge K. F. A selective motility medium for routine isolation of Salmonella. J Hyg (Lond) 1969 Jun;67(2):181–186. doi: 10.1017/s0022172400041589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harvey R. W., Mahabir D. E., Price T. H. A method of secondary enrichment for salmonellas independent of selectively toxic chemicals. J Hyg (Lond) 1966 Sep;64(3):361–366. doi: 10.1017/s002217240004064x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JAMESON J. E. A discussion of the dynamics of Salmonella enrichment. J Hyg (Lond) 1962 Jun;60:193–207. doi: 10.1017/s0022172400039462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kafel S., Bryan F. L. Effects of enrichment media and incubation conditions on isolating salmonellae from ground-meat filtrate. Appl Environ Microbiol. 1977 Sep;34(3):285–291. doi: 10.1128/aem.34.3.285-291.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. STUART P. F., PIVNICK H. ISOLATION OF SALMONELLAE BY SELECTIVE MOTILITY SYSTEMS. Appl Microbiol. 1965 May;13:365–372. doi: 10.1128/am.13.3.365-372.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smyser C. F., Snoeyenbos G. H., McKie B. Isolation of salmonellae from rendered by-products and poultry litter cultured in enrichment media incubated at elevated temperatures. Avian Dis. 1970 May;14(2):248–254. [PubMed] [Google Scholar]
  12. Thomason B. M., Dodd D. J., Cherry W. B. Increased recovery of salmonellae from environmental samples enriched with buffered peptone water. Appl Environ Microbiol. 1977 Sep;34(3):270–273. doi: 10.1128/aem.34.3.270-273.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Trichopoulos D., Papadakis J. A., Karalis D., Vassilliadis P. Incubation at raised temperature or enrichment media, combined with secondary enrichment in Rappaport's medium, for the isolation of salmonellas from sewage. J Hyg (Lond) 1975 Apr;74(2):205–213. doi: 10.1017/s0022172400024268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vassiliadis P., Papadakis J. A., Karalis D., Trichopoulos D. Enrichment in Muller-Kauffmann's broth and Rappaport's broth from buffered peptone water in the isolation of salmonellae from minced meat. J Appl Bacteriol. 1976 Jun;40(3):349–354. doi: 10.1111/j.1365-2672.1976.tb04183.x. [DOI] [PubMed] [Google Scholar]

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