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. 1979 May;37(5):1041–1043. doi: 10.1128/aem.37.5.1041-1043.1979

Genetic Evidence for Plasmid-Linked Lactose Metabolism in Streptococcus lactis subsp. diacetylactis†

G M Kempler 1, L L McKay 1
PMCID: PMC243345  PMID: 16345374

Abstract

It has been previously observed that loss of plasmid pGK4101 occurred concomitantly with loss of lactose-fermenting ability in Streptococcus lactis subsp. diacetylactis 18-16. Transfer of this 41-megadalton plasmid to LM0230, a lactosenegative (Lac−) strain of S. lactis, required cell-to-cell contact and resulted in a conversion of LM0230 to the Lac+ phenotype. This confirms the linkage of lactose-fermenting ability to the 41-megadalton plasmid in S. lactis subsp. diacetylactis and, in addition, demonstrates transfer by a process resembling conjugation in the group N streptococci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dunny G. M., Clewell D. B. Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid. J Bacteriol. 1975 Nov;124(2):784–790. doi: 10.1128/jb.124.2.784-790.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Jacob A. E., Douglas G. J., Hobbs S. J. Self-transferable plasmids determining the hemolysin and bacteriocin of Streptococcus faecalis var. zymogenes. J Bacteriol. 1975 Mar;121(3):863–872. doi: 10.1128/jb.121.3.863-872.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jacob A. E., Hobbs S. J. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. doi: 10.1128/jb.117.2.360-372.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Klaenhammer T. R., McKay L. L., Baldwin K. A. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid. Appl Environ Microbiol. 1978 Mar;35(3):592–600. doi: 10.1128/aem.35.3.592-600.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LeBlanc D. J., Hawley R. J., Lee L. N., St Martin E. J. "Conjugal" transfer of plasmid DNA among oral streptococci. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3484–3487. doi: 10.1073/pnas.75.7.3484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McKay L. L., Baldwin K. A., Efstathiou J. D. Transductional evidence for plasmid linkage of lactose metabolism in streptococcus lactis C2. Appl Environ Microbiol. 1976 Jul;32(1):45–52. doi: 10.1128/aem.32.1.45-52.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McKay L. L., Baldwin K. A. Simultaneous loss of proteinase- and lactose-utilizing enzyme activities in Streptococcus lactis and reversal of loss by transduction. Appl Microbiol. 1974 Sep;28(3):342–346. doi: 10.1128/am.28.3.342-346.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McKay L. L., Baldwin K. A. Stabilization of Lactose Metabolism in Streptococcus lactis C2. Appl Environ Microbiol. 1978 Aug;36(2):360–367. doi: 10.1128/aem.36.2.360-367.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McKay L. L., Baldwin K. A., Zottola E. A. Loss of lactose metabolism in lactic streptococci. Appl Microbiol. 1972 Jun;23(6):1090–1096. doi: 10.1128/am.23.6.1090-1096.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKay L. L., Cords B. R., Baldwin K. A. Transduction of lactose metabolism in Streptococcus lactis C2. J Bacteriol. 1973 Sep;115(3):810–815. doi: 10.1128/jb.115.3.810-815.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pearce L. E., Skipper N. A., Jarvis B. D. Proteinase activity in slow lactic acid-producing variants of Streptococcus lactis. Appl Microbiol. 1974 May;27(5):933–937. doi: 10.1128/am.27.5.933-937.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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