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. 1979 Jun;37(6):1079–1084. doi: 10.1128/aem.37.6.1079-1084.1979

Macromolecular composition of a Cellulomonas sp. cultivated in continuous culture under glucose and zinc limitation.

R J Summers, V R Srinivasan
PMCID: PMC243357  PMID: 114114

Abstract

The mutant strain of Cellulomonas sp. (ATCC 21399) was cultivated under glucose and zinc limitation at a variety of growth rates in continuous culture. The growth characteristics and macromolecular composition of the population varied with the limitation imposed and the growth rate. Glucose- and zinc-limited cultures maintained a constant relative protein content. The relative ribonucleic acid content increased, whereas the carbohydrate and deoxyribonucleic acid contents decreased with an increase in the population growth rate in glucose-limited cultures. Free unbound lipid remained constant. The maximum population growth rate in zinc-limited cultures was directly proportional to the zinc concentration and demonstrated a traditional steady-state function. The nucleic acid content increased with increased growth rate; however, the relative nucleic acid content was significantly depressed when compared to glucose limited cells. This manner of cultivation may prove to be a useful tool for the production of single cell protein with lowered nucleic acid content and the elucidation of micronutrient involvement in growth-related processes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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