Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1979 Jun;37(6):1193–1195. doi: 10.1128/aem.37.6.1193-1195.1979

Plasmid Profiles of Lactose-Negative and Proteinase-Deficient Mutants of Streptococcus lactis C10, ML3, and M18 †

Steve A Kuhl 1, L D Larsen 1, L L McKay 1
PMCID: PMC243377  PMID: 16345399

Abstract

Lactose- and proteinase-negative (Lac− Prt−) mutants of Streptococcus lactis C10, ML3, and M18 were isolated after treatment with ethidium bromide. The Lac− Prt− mutants of C10 were missing a 40-megadalton plasmid. A 33-megadalton plasmid was absent in the ML3 mutants, and the M18 variants lacked a 45-megadalton plasmid. The results suggest a linkage of these metabolic traits to the respective plasmids. The possible complexity of the interrelationship between lactose metabolism and proteinase activity is presented.

Full text

PDF
1193

Images in this article

1194Image
on p.1194

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Plasmids, loss of lactose metabolism, and appearance of partial and full lactose-fermenting revertants in Streptococcus cremoris B1. J Bacteriol. 1977 Jan;129(1):367–377. doi: 10.1128/jb.129.1.367-377.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CITTI J. E., SANDINE W. E., ELLIKER P. R. COMPARISON OF SLOW AND FAST ACID-PRODUCING STREPTOCOCCUS LACTIS. J Dairy Sci. 1965 Jan;48:14–18. doi: 10.3168/jds.s0022-0302(65)88152-8. [DOI] [PubMed] [Google Scholar]
  3. Efstathiou J. D., McKay L. L. Plasmids in Streptococcus lactis: evidence that lactose metabolism and proteinase activity are plasmid linked. Appl Environ Microbiol. 1976 Jul;32(1):38–44. doi: 10.1128/aem.32.1.38-44.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kempler G. M., McKay L. L. Characterization of Plasmid Deoxyribonucleic Acid in Streptococcus lactis subsp. diacetylactis: Evidence for Plasmid-Linked Citrate Utilization. Appl Environ Microbiol. 1979 Feb;37(2):316–323. doi: 10.1128/aem.37.2.316-323.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klaenhammer T. R., McKay L. L., Baldwin K. A. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid. Appl Environ Microbiol. 1978 Mar;35(3):592–600. doi: 10.1128/aem.35.3.592-600.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Larsen L. D., McKay L. L. Isolation and characterization of plasmid DNA in Streptococcus cremoris. Appl Environ Microbiol. 1978 Dec;36(6):944–952. doi: 10.1128/aem.36.6.944-952.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McKay L. L., Baldwin K. A., Efstathiou J. D. Transductional evidence for plasmid linkage of lactose metabolism in streptococcus lactis C2. Appl Environ Microbiol. 1976 Jul;32(1):45–52. doi: 10.1128/aem.32.1.45-52.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McKay L. L., Baldwin K. A. Plasmid distribution and evidence for a proteinase plasmid in Streptococcus lactis C2-1. Appl Microbiol. 1975 Apr;29(4):546–548. doi: 10.1128/am.29.4.546-548.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McKay L. L., Baldwin K. A. Simultaneous loss of proteinase- and lactose-utilizing enzyme activities in Streptococcus lactis and reversal of loss by transduction. Appl Microbiol. 1974 Sep;28(3):342–346. doi: 10.1128/am.28.3.342-346.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKay L. L., Baldwin K. A., Zottola E. A. Loss of lactose metabolism in lactic streptococci. Appl Microbiol. 1972 Jun;23(6):1090–1096. doi: 10.1128/am.23.6.1090-1096.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McKay L., Miller A., 3rd, Sandine W. E., Elliker P. R. Mechanisms of lactose utilization by lactic acid streptococci: enzymatic and genetic analyses. J Bacteriol. 1970 Jun;102(3):804–809. doi: 10.1128/jb.102.3.804-809.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pearce L. E., Skipper N. A., Jarvis B. D. Proteinase activity in slow lactic acid-producing variants of Streptococcus lactis. Appl Microbiol. 1974 May;27(5):933–937. doi: 10.1128/am.27.5.933-937.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Westhoff D. C., Cowman R. A., Swaisgood H. E. Characterization of an intracellular proteinase of a slow acid producing mutant of Streptococcus lactis. J Dairy Sci. 1971 Sep;54(9):1259–1264. doi: 10.3168/jds.S0022-0302(71)86017-4. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES