Abstract
Determinations of microbial populations were carried out by using a new electrode system composed of two electrodes. Each electrode was constructed from a platinum anode and a silver peroxide cathode. The anode of the reference electrode was covered with cellulose dialysis membrane. The response time of the electrode system was 15 min in culture broth, and current differences between the two electrodes were proportional to populations of microbial cells in cultures of Saccharomyces cerevisiae and Lactobacillus fermentum. Current differences were reproducible; the average relative error was 5%. Furthermore, cell populations of S. cerevisiae in a fermentor could be continuously estimated by using this electrochemical method.
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Selected References
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- Hardy D., Kraeger S. J., Dufour S. W., Cady P. Rapid detection of microbial contamination in frozen vegetables by automated impedance measurements. Appl Environ Microbiol. 1977 Jul;34(1):14–17. doi: 10.1128/aem.34.1.14-17.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kagan R. L., Schuette W. H., Zierdt C. H., MacLowry J. D. Rapid automated disgnosis of bacteremia by impedance detection. J Clin Microbiol. 1977 Jan;5(1):51–57. doi: 10.1128/jcm.5.1.51-57.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pringle J. R., Mor J. R. Methods for monitoring the growth of yeast cultures and for dealing with the clumping problem. Methods Cell Biol. 1975;11:131–168. doi: 10.1016/s0091-679x(08)60320-9. [DOI] [PubMed] [Google Scholar]
- Wilkins J. R., Stoner G. E., Boykin E. H. Microbial detection method based on sensing molecular hydrogen. Appl Microbiol. 1974 May;27(5):949–952. doi: 10.1128/am.27.5.949-952.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkins J. R., Young R. N., Boykin E. H. Multichannel electrochemical microbial detection unit. Appl Environ Microbiol. 1978 Jan;35(1):214–215. doi: 10.1128/aem.35.1.214-215.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zafari Y., Martin W. J. Comparison of the BACTOMETER microbial monitoring system with conventional methods for detection of microorganisms in urine specimens. J Clin Microbiol. 1977 May;5(5):545–547. doi: 10.1128/jcm.5.5.545-547.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]