Figure 2.

Murine CD40L-activated B cells exhibit a similar co-regulatory molecule phenotype as dendritic cells. Mature DC were generated from murine bone marrow by culture with rIL-4 and rGM-CSF for 4 days followed by a two day culture with rTNF-α. CD40L-activated B cells were generated by culture for 12 days on monolayers of L cells transfected with human CD40L in the presence of rIL-4 and cyclosporin A. Cells were phenotyped using a combination of B220-specific antibody and antibody specific for MHC I, MHC II, CD80, or CD86. Co-regulatory molecule expression (solid histograms) on cells gated for B220 expression is presented. Open histograms represent data generated with FITC-isotype control antibodies. Data from a representative experiment (>5 total) are presented.