Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1979 Aug;38(2):200–204. doi: 10.1128/aem.38.2.200-204.1979

Attached Growth of Sphaerotilus natans in Continuous-Flow Apparatus and Its Growth Inhibition by 9-β-d-Arabinofuranosyladenine

Hiroji Yoshikawa 1, Yo Takiguchi 1
PMCID: PMC243465  PMID: 16345420

Abstract

Sphaerotilus natans grew at the maximum specific growth rate (μmax) of 0.43/h when cultivated on PGY medium at 25°C. The organism mainly grew attached to inside of the culture vessels when the culture medium was fed to the completely mixed continuous-flow apparatus at a dilution rate above μmax and the attached growth was directly related to the dilution rate. When a low concentration of the medium was supplied to the apparatus, almost all of the cells grown were filamentous and attached to the inside of the vessels. When a high concentration of the medium was fed, the organism grew as single cells or short chains and flowed out into the effluent. The attached growth of S. natans in the continuous-flow apparatus was inhibited by the minimal inhibitory concentration of 0.5 to 1.0 μg of 9-β-d-arabinofuranosyladenine per ml. 9-β-d-Arabinofuranosyladenine showed bacteriocidal activity against S. natans at a concentration of 50 to 100 μg/ml.

Full text

PDF
200

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armbruster E. H. Improved technique for isolation and identification of Sphaerotilus. Appl Microbiol. 1969 Feb;17(2):320–321. doi: 10.1128/am.17.2.320-321.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DONDERO N. C., PHILLIPS R. A., HEUKELEKIAN H. Isolation and preservation of cultures of Sphaerotilus. Appl Microbiol. 1961 May;9:219–227. doi: 10.1128/am.9.3.219-227.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DONDERO N. C. Sphaerotilus, its nature and economic significance. Adv Appl Microbiol. 1961;3:77–107. doi: 10.1016/s0065-2164(08)70507-0. [DOI] [PubMed] [Google Scholar]
  4. Dias F. F., Heukelekian H. Utilization of Inorganic Nitrogen Compounds by Sphaerotilus natans Growing in a Continuous-Flow Apparatus. Appl Microbiol. 1967 Sep;15(5):1083–1086. doi: 10.1128/am.15.5.1083-1086.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dondero N. C. The Sphaerotilus-Leptothrix group. Annu Rev Microbiol. 1975;29:407–428. doi: 10.1146/annurev.mi.29.100175.002203. [DOI] [PubMed] [Google Scholar]
  6. Takiguchi Y., Yoshikawa H., Terao M. Selective Growth Inhibition of Sphaerotilus natans and Beggiatoa sp. by Nucleosides. Appl Environ Microbiol. 1978 Nov;36(5):658–661. doi: 10.1128/aem.36.5.658-661.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES