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. 1979 Aug;38(2):212–215. doi: 10.1128/aem.38.2.212-215.1979

Automated Enzymatic Method for the Determination of Ammonia: Application to Rumen Fluid, Gut Fluid, and Plasma

Albert L Baetz 1, Terry D Faidley 1, Milton J Allison 1
PMCID: PMC243467  PMID: 16345421

Abstract

The enzymatic method for the determination of ammonia with glutamate dehydrogenase has been adapted to the AutoAnalyzer. The automated method was applicable for NH3 measurement in sheep rumen samples, swine gut fluid samples, and bovine plasma. Results were compared with those of the Conway diffusion method and the manual enzymatic method. The automated method allows 30 samples per h to be analyzed routinely. Values were comparable with those with the manual method, but lower than those with the Conway diffusion method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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