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. 1979 Aug;38(2):248–257. doi: 10.1128/aem.38.2.248-257.1979

Comparison of antibodies in marine fish from clean and polluted waters of the New York Bight: relative levels against 36 bacteria.

R A Robohm, C Brown, R A Murchelano
PMCID: PMC243474  PMID: 518084

Abstract

Fish from polluted waters are subject to increased prevalence of disease. Because they respond to bacterial pathogens by producing serum antibodies, it was possible to construct a seasonal serological record in three fish species from clean and polluted waters of the New York Bight. Antibody levels were determined by testing sera for agglutinating activity against 36 strains of bacteria. Evaluation of 5,100 antibody titrations showed the following. During warm months, summer flounder (Paralichthys dentatus) from the polluted area had significantly higher antibody levels and antibody to a greater diversity of bacteria than fish from the unpolluted area. Weakfish (Cynoscion regalis) from the same polluted area shared with summer flounder raised titers to many bacteria. The greatest proportion of raised titers was against Vibrio species, although prominent titers were also seen against Aeromonas salmonicida and Haemophilus piscium, bacteria usually associated with diseases in freshwater but not marine fish. Differences between polluted and clean waters were not as evident in winter flounder (Pseudopleuronectes americanus) during cold months. This could be due, in part, to reduced antibody production at colder temperatures. The data illustrate the usefulness of the serum antibody record in identifying environmental exposure to bacteria in marine fish and indicate that the polluted New York Bight apex has increased levels and diversity of bacteria during warm months.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amend D. F., Fender D. C. Uptake of bovine serum albumin by rainbow trout from hypersmotic solutions: a model for vaccinating fish. Science. 1976 May 21;192(4241):793–794. doi: 10.1126/science.1265480. [DOI] [PubMed] [Google Scholar]
  2. Avtalion R. R., Wojdani A., Malik Z., Shahrabani R., Duczyminer M. Influence of environmental temperature on the immune response in fish. Curr Top Microbiol Immunol. 1973;61:1–35. doi: 10.1007/978-3-642-65531-9_1. [DOI] [PubMed] [Google Scholar]
  3. Baross J., Liston J. Occurrence of Vibrio parahaemolyticus and related hemolytic vibrios in marine environments of Washington State. Appl Microbiol. 1970 Aug;20(2):179–186. doi: 10.1128/am.20.2.179-186.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Colwell R. R., Kaper J., Joseph S. W. Vibrio cholerae, Vibrio parahaemolyticus, and other vibrios: occurrence and distribution in Chesapeake Bay. Science. 1977 Oct 28;198(4315):394–396. [PubMed] [Google Scholar]
  5. Evelyn T. P. The agglutinin response in sockeye salmon vaccinated intraperitoneally with a heat-killed preparation of the bacterium responsible for salmonid kidney disease. J Wildl Dis. 1971 Oct;7(4):328–335. [PubMed] [Google Scholar]
  6. Janssen W. A., Meyers C. D. Fish: serologic evidence of infection with human pathogens. Science. 1968 Feb 2;159(3814):547–548. doi: 10.1126/science.159.3814.547. [DOI] [PubMed] [Google Scholar]
  7. KRANTZ G. E., REDDECLIFF J. M., HEIST C. E. DEVELOPMENT OF ANTIBODIES AGAINST AEROMONAS SALMONICIDA IN TROUT. J Immunol. 1963 Dec;91:757–760. [PubMed] [Google Scholar]
  8. Kaneko T., Colwell R. R. Ecology of Vibrio parahaemolyticus in Chesapeake Bay. J Bacteriol. 1973 Jan;113(1):24–32. doi: 10.1128/jb.113.1.24-32.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keith L., Beamer P., Hazdra J. J., Nair V., Callaghan O. Chemical pollutants in relation to diseases in fish. Ann N Y Acad Sci. 1978 Sep 29;298:535–546. doi: 10.1111/j.1749-6632.1977.tb19288.x. [DOI] [PubMed] [Google Scholar]
  10. Mearns A. J., Sherwood M. J. Distribution of neoplasms and other diseases in marine fishes relative to the discharge of waste water. Ann N Y Acad Sci. 1978 Sep 29;298:210–224. doi: 10.1111/j.1749-6632.1977.tb19266.x. [DOI] [PubMed] [Google Scholar]
  11. Scott M. The pathogenicity of Aeromonas salmonicida (Griffin) in sea and brackish waters. J Gen Microbiol. 1968 Feb;50(2):321–327. doi: 10.1099/00221287-50-2-321. [DOI] [PubMed] [Google Scholar]
  12. Sherwood M. J., Mearns A. J. Environmental significance of fin erosion in southern California demersal fishes. Ann N Y Acad Sci. 1978 Sep 29;298:177–189. doi: 10.1111/j.1749-6632.1977.tb19263.x. [DOI] [PubMed] [Google Scholar]
  13. Shotts E. B., Jr, Gaines J. L., Jr, Martin L., Prestwood A. K. Aeromonas-induced deaths among fish and reptiles in an eutrophic inland lake. J Am Vet Med Assoc. 1972 Sep 15;161(6):603–607. [PubMed] [Google Scholar]
  14. Trump G. N., Hildemann W. H. Antibody responses of goldfish to bovine serum albumin. Primary and secondary responses. Immunology. 1970 Oct;19(4):621–627. [PMC free article] [PubMed] [Google Scholar]

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