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. 1979 Aug;38(2):297–300. doi: 10.1128/aem.38.2.297-300.1979

Noncorrelation between mouse toxicity and serologically assayed toxin in Clostridium botulinum type A culture fluids.

M J Betley, H Sugiyama
PMCID: PMC243480  PMID: 391153

Abstract

Toxicity in culture fluids of several Clostridium botulinum type A strains was assayed in mice and converted to weight equivalent. The toxin-related antigen in the samples was quantitated by a radioimmunoassay which used standards of known antigen concentration instead of the usually used toxicity. Freshly prepared samples had reasonably similar titers of toxin and antigen. When the samples were held at room temperature for several weeks, toxicity decreased more than antigenicity, but the relative decreases of the two varied with the samples. The results are discussed as evidence that serological assays of botulinum toxin cannot always be used for accurate determination of toxicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boroff D. A., Chu-Chen G. Radioimmunoassay for type A toxin of Clostridium botulinum. Appl Microbiol. 1973 Apr;25(4):545–549. doi: 10.1128/am.25.4.545-549.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DasGupta B. R., Sugiyama H. A common subunit structure in Clostridium botulinum type A, B and E toxins. Biochem Biophys Res Commun. 1972 Jul 11;48(1):108–112. doi: 10.1016/0006-291x(72)90350-6. [DOI] [PubMed] [Google Scholar]
  3. DasGupta B. R., Sugiyama H. Inhibition of Clostridium botulinum types A and B hemagglutinins by sugars. Can J Microbiol. 1977 Sep;23(9):1257–1260. doi: 10.1139/m77-188. [DOI] [PubMed] [Google Scholar]
  4. Habermann E. 125I-labeled neurotoxin from Clostridium botulinum A: preparation, binding to synaptosomes and ascent to the spinal cord. Naunyn Schmiedebergs Arch Pharmacol. 1974;281(1):47–56. doi: 10.1007/BF00500611. [DOI] [PubMed] [Google Scholar]
  5. Haustein D. Effective radioiodination by lactoperoxidase and solubilisation of cell-surface proteins of cultured murine T lymphoma cells. J Immunol Methods. 1975 Apr;7(1):25–38. doi: 10.1016/0022-1759(75)90127-1. [DOI] [PubMed] [Google Scholar]
  6. Johnson H. M., Brenner K., Angelotti R., Hall H. E. Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation. J Bacteriol. 1966 Mar;91(3):967–974. doi: 10.1128/jb.91.3.967-974.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Knox J., Brown W. P., Spero L. The role of sulfhydryl groups in the activity of type A botulinum toxin. Biochim Biophys Acta. 1970 Aug 21;214(2):350–354. doi: 10.1016/0005-2795(70)90012-7. [DOI] [PubMed] [Google Scholar]
  8. Mestrandrea L. W. Rapid detection of Clostridium botulinum toxin by capillary tube diffusion. Appl Microbiol. 1974 Jun;27(6):1017–1022. doi: 10.1128/am.27.6.1017-1022.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller B. A., Reiser R. F., Bergdoll M. S. Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent. Appl Environ Microbiol. 1978 Sep;36(3):421–426. doi: 10.1128/aem.36.3.421-426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moberg L. J., Sugiyama H. Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex. Appl Environ Microbiol. 1978 May;35(5):878–880. doi: 10.1128/aem.35.5.878-880.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NOELKEN M. E., NELSON C. A., BUCKLEY C. E., 3rd, TANFORD C. GROSS CONFORMATION OF RABBIT 7 S GAMMA-IMMUNOGLOBULIN AND ITS PAPAIN-CLEAVED FRAGMENTS. J Biol Chem. 1965 Jan;240:218–224. [PubMed] [Google Scholar]
  12. Notermans S., Dufrenne J., Schothorst M. Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A. Jpn J Med Sci Biol. 1978 Feb;31(1):81–85. doi: 10.7883/yoken1952.31.81. [DOI] [PubMed] [Google Scholar]
  13. Rodbard D., Rayford P. L., Cooper J. A., Ross G. T. Statistical quality control of radioimmunoassays. J Clin Endocrinol Metab. 1968 Oct;28(10):1412–1418. doi: 10.1210/jcem-28-10-1412. [DOI] [PubMed] [Google Scholar]
  14. SINITSYN V. A. [Utilization of the indirect hemagglutination reaction for indication of botulism toxins. Part II. Modification of the method of indirect hemagglutination reaction and its comparative evaluation with various tests used for detection of botulism toxins]. Zh Mikrobiol Epidemiol Immunobiol. 1960 Apr;31:102–107. [PubMed] [Google Scholar]
  15. Sakaguchi G., Sakaguchi S., Kozaki S., Sugii S., Oishi I. Cross reaction in reversed passive hemagglutination between Clostridium botulinum type A and B toxins and its avoidance by the sue of anti-toxic component immunoglobulin isolated by affinity chromatography. Jpn J Med Sci Biol. 1974 Jun;27(3):161–172. doi: 10.7883/yoken1952.27.161. [DOI] [PubMed] [Google Scholar]
  16. Sugiyama H., Moberg L. J., Messer S. L. Improved procedure for crystallization of Clostridium botulinum type A toxic complexes. Appl Environ Microbiol. 1977 Apr;33(4):963–966. doi: 10.1128/aem.33.4.963-966.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sugiyama H., Oishi I., Dasgupta B. R. Evaluation of type A botulinal toxin assays that use antitoxin to crystalline toxin. Appl Microbiol. 1974 Feb;27(2):333–336. doi: 10.1128/am.27.2.333-336.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vermilyea B. L., Walker H. W., Ayres J. C. Detection of botulinal toxins by immunodiffusion. Appl Microbiol. 1968 Jan;16(1):21–24. doi: 10.1128/am.16.1.21-24.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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