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. 1979 Sep;38(3):351–358. doi: 10.1128/aem.38.3.351-358.1979

Comparison of m-Endo LES, MacConkey, and Teepol media for membrane filtration counting of total coliform bacteria in water.

W O Grabow, M du Preez
PMCID: PMC243498  PMID: 394678

Abstract

Total coliform counts obtained by means of standard membrane filtration techniques, using MacConkey agar, m-Endo LES agar, Teepol agar, and pads saturated with Teepol broth as growth media, were compared. Various combinations of these media were used in tests on 490 samples of river water and city wastewater after different stages of conventional purification and reclamation processes including lime treatment, and filtration, active carbon treatment, ozonation, and chlorination. Endo agar yielded the highest average counts for all these samples. Teepol agar generally had higher counts then Teepol broth, whereas MacConkey agar had the lowest average counts. Identification of 871 positive isolates showed that Aeromonas hydrophila was the species most commonly detected. Species of Escherichia, Citrobacter, Klebsiella, and Enterobacter represented 55% of isolates which conformed to the definition of total coliforms on Endo agar, 54% on Teepol agar, and 45% on MacConkey agar. Selection for species on the media differed considerably. Evaluation of these data and literature on alternative tests, including most probable number methods, indicated that the technique of choice for routine analysis of total coliform bacteria in drinking water is membrane filtration using m-Endo LES agar as growth medium without enrichment procedures or a cytochrome oxidase restriction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagley S. T., Seidler R. J. Significance of fecal coliform-positive Klebsiella. Appl Environ Microbiol. 1977 May;33(5):1141–1148. doi: 10.1128/aem.33.5.1141-1148.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burman N. P., Colbourne J. S. Techniques for the assessment of growth of micro-organisms on plumbing materials used in contact with potable water supplies. J Appl Bacteriol. 1977 Aug;43(1):137–144. doi: 10.1111/j.1365-2672.1977.tb00732.x. [DOI] [PubMed] [Google Scholar]
  3. Dutkaand B. J., Tobin S. E. Study on the efficiency of four procedures for enumerating coliforms in water. Can J Microbiol. 1976 May;22(5):630–635. doi: 10.1139/m76-093. [DOI] [PubMed] [Google Scholar]
  4. Echeverria P., Verhaert L., Ulyangco C. V., Komalarini S., Ho M. T., Orskov F., Orskov I. Antimicrobial resistance and enterotoxin production among isolates of Escherichia coli in the Far East. Lancet. 1978 Sep 16;2(8090):589–592. doi: 10.1016/s0140-6736(78)92820-9. [DOI] [PubMed] [Google Scholar]
  5. Favero M. S., Petersen N. J., Carson L. A., Bond W. W., Hindman S. H. Gram-negative water bacteria in hemodialysis systems. Health Lab Sci. 1975 Oct;12(4):321–334. [PubMed] [Google Scholar]
  6. Fliermans C. B., Gorden R. W., Hazen T. C., Esch G. W. Aeromonas distribution and survival in a thermally altered lake. Appl Environ Microbiol. 1977 Jan;33(1):114–122. doi: 10.1128/aem.33.1.114-122.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grabow W. O., Middendorff I. G., Basson N. C. Role of lime treatment in the removal of bacteria, enteric viruses, and coliphages in a wastewater reclamation plant. Appl Environ Microbiol. 1978 Apr;35(4):663–669. doi: 10.1128/aem.35.4.663-669.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayek L. J., Willis G. W. A comparison of two commerical methods for the identification of the Enterobacteriaceae--API 20E and the Enterotube--with conventional methods. J Clin Pathol. 1976 Feb;29(2):158–161. doi: 10.1136/jcp.29.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hazen T. C., Fliermans C. B., Hirsch R. P., Esch G. W. Prevalence and distribution of Aeromonas hydrophila in the United States. Appl Environ Microbiol. 1978 Nov;36(5):731–738. doi: 10.1128/aem.36.5.731-738.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Highsmith A. K., Feeley J. C., Skaliy P., Wells J. G., Wood B. T. Isolation of Yersinia enterocolitica from well water and growth in distilled water. Appl Environ Microbiol. 1977 Dec;34(6):745–750. doi: 10.1128/aem.34.6.745-750.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lin S. Evaluation of coliform tests for chlorinated secondary effluents. J Water Pollut Control Fed. 1973 Mar;45(1):498–506. [PubMed] [Google Scholar]
  12. Lupo L., Strickland E., Dufour A., Cabelli V. The effect of oxidase positive bacteria on total coliform density estimates. Health Lab Sci. 1977 Apr;14(2):117–121. [PubMed] [Google Scholar]
  13. Robertson E. A., MacLowry J. D. Mathematical analysis of the API enteric 20 profile register using a computer diagnostic model. Appl Microbiol. 1974 Oct;28(4):691–695. doi: 10.1128/am.28.4.691-695.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rosenberg M. L., Koplan J. P., Wachsmuth I. K., Wells J. G., Gangarosa E. J., Guerrant R. L., Sack D. A. Epidemic diarrhea at Crater Lake from enterotoxigenic Escherichia coli. A large waterborne outbreak. Ann Intern Med. 1977 Jun;86(6):714–718. doi: 10.7326/0003-4819-86-6-714. [DOI] [PubMed] [Google Scholar]
  15. Schiemann D. A. Isolation of Yersinia enterocolitica from surface and well waters in Ontario. Can J Microbiol. 1978 Sep;24(9):1048–1052. doi: 10.1139/m78-173. [DOI] [PubMed] [Google Scholar]
  16. Schiemann D. A., Manley J. L., Arnold M. O. Coliphage interference in the recovery of coliform bacteria. J Water Pollut Control Fed. 1976 Mar;48(3 Pt 1):533–539. [PubMed] [Google Scholar]
  17. Tobin R. S., Dutka B. J. Comparison of the surface structure, metal binding, and fecal coliform recoveries of nine membrane filters. Appl Environ Microbiol. 1977 Jul;34(1):69–79. doi: 10.1128/aem.34.1.69-79.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van der Kooij D. The occurrence of Pseudomonas spp. in surface water and in tap water as determined on citrate media. Antonie Van Leeuwenhoek. 1977;43(2):187–197. doi: 10.1007/BF00395673. [DOI] [PubMed] [Google Scholar]

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