Table 1.

Dissociation Constants for Synthetic and Expressed wt bZIP Bound to DNA Sites

	Kd (10-9 M)a
binding site	synthetic wt bZIP	expressed wt bZIPb
WT AP-1	13 ± 0.38
AP-1	13 ± 0.59e	4.8 ± 0.12b
CRE	12 ± 0.28
C/EBP	120 ± 16e	29 ± 3.6b
XRE1	240 ± 18e
Arnt E-box	570 ± 0.69c,e
Max E-box	840 ± 26c
HRE	1400 ± 160c,e
Partial	280 ± 49e
AP-1 half	84 ± 0.01	23 ± 8.7b
C/EBP half	>5000c	>1500c
Arnt E-box half	>4000c
Max E-box Half	no activityd
NS	no activityd	no activityd

^aAverage values of dissociation constants were obtained from two independent experiments; R values >0.97.

^bHigher-order bandshifts were not included in calculation of dissociation constants (see text and Supporting Information).

^cSaturation protein binding was not achieved in these titrations.

^dThe concentration of wt bZIP ranged from 0 to 1000 nM.

^eFrom ref 17.