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. 1979 Sep;38(3):554–556. doi: 10.1128/aem.38.3.554-556.1979

General Properties of Beta-Galactosidase of Xanthomonas campestris

Joseph F Frank 1, George A Somkuti 1
PMCID: PMC243532  PMID: 16345439

Abstract

Partially purified β-galactosidase of Xanthomonas campestris required 32 to 37°C and pH 5.5 to 5.8 for optimum activity. The enzyme had low affinity for lactose hydrolysis (Km = 22 mM) and was inhibited by thiol group reagents, ethylenediaminetetraacetic acid, galactose, and d-galactal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANEMA P. J. PURIFICATION AND SOME PROPERTIES OF BETA-GLACTOSIDASE OF BACILLUS SUBTILIS. Biochim Biophys Acta. 1964 Sep 18;89:495–502. [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hidalgo C., Reyes J., Goldschmidt R. Induction and general properties of beta-galactosidase and beta-galactoside permease in Pseudomonas BAL-31. J Bacteriol. 1977 Feb;129(2):821–829. doi: 10.1128/jb.129.2.821-829.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LANDMAN O. E. Properties and induction of beta-galactosidase in Bacillus megaterium. Biochim Biophys Acta. 1957 Mar;23(3):558–569. doi: 10.1016/0006-3002(57)90377-3. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lee Y. C. Inhibition of beta-D-galactosidases by D-galactal. Biochem Biophys Res Commun. 1969 Apr 10;35(1):161–167. doi: 10.1016/0006-291x(69)90499-9. [DOI] [PubMed] [Google Scholar]
  8. McFeters G. A., Sandine W. E., Elliker P. R. Involvement of sulfhydryl groups in the -galactosidase of Streptococcus lactis. J Bacteriol. 1971 Oct;108(1):599–600. doi: 10.1128/jb.108.1.599-600.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Premi L., Sandine W. E., Elliker P. R. Lactose-hydrolyzing enzymes of Lactobacillus species. Appl Microbiol. 1972 Jul;24(1):51–57. doi: 10.1128/am.24.1.51-57.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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