Abstract
Investigations on farms where botulism has occurred in cows showed that proteolytic Clostridium botulinum type B was present in newly made grass silages. Experiments were undertaken to study growth and toxin production of C. botulinum in grass. Of the strains tested only proteolytic strains of C. botulinum types A and B were able to produce toxin with grass as a substrate. Proteolytic strains of type B produced both medium (12S) and large (16S) toxin forms. The minimal water activity (aw) for toxin production at pH 6.5 and 5.8 was 0.94. At pH 5.3, toxin was produced at an aw of 0.985. These results indicate that proteolytic strains of C. botulinum (if present) may multiply and produce toxin in wilted grass silages.
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Selected References
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- Baird-Parker A. C., Freame B. Combined effect of water activity, pH and temperature on the growth of Clostridium botulinum from spore and vegetative cell inocula. J Appl Bacteriol. 1967 Dec;30(3):420–429. doi: 10.1111/j.1365-2672.1967.tb00320.x. [DOI] [PubMed] [Google Scholar]
- Boroff D. A., Fleck U. Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum. J Bacteriol. 1966 Nov;92(5):1580–1581. doi: 10.1128/jb.92.5.1580-1581.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Breukink H. J., Wagenaar G., Wensing T., Notermans S., Poulos P. W. Voedselvergiftiging bij runderen veroorzaakt door het eten van bierbostel besmet met Clostridium botulinum type B. Tijdschr Diergeneeskd. 1978 Mar 15;103(6):303–311. [PubMed] [Google Scholar]
- Doutre M. P. Fréquence au Sénégal du botulisme animal d'origine hydrique. Rev Elev Med Vet Pays Trop. 1969;22(1):29–31. [PubMed] [Google Scholar]
- Gruys E., Binkhorst G. J., van den Bercken J., Meijers P., Haagsma J. Botulisme bij enkele pinken. Tijdschr Diergeneeskd. 1977 Aug 15;102(16):983–988. [PubMed] [Google Scholar]
- Kozaki S., Sakaguchi S., Sakaguchi G. Purification and some properties of progenitor toxins of Clostridium botulinum type B. Infect Immun. 1974 Oct;10(4):750–756. doi: 10.1128/iai.10.4.750-756.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Notermans S., Breukink H. J., Wensing T., Wagenaar G. Voorkomen van Clostridium botulinum in pensinhoud en faeces van runderen gevoederd met bostel welke natuurlijk besmet was met Clostridium botulinum. Tijdschr Diergeneeskd. 1978 Dec 15;103(24):1327–1333. [PubMed] [Google Scholar]
- Ohishi I., Sugii S., Sakaguchi G. Oral toxicities of Clostridium botulinum toxins in response to molecular size. Infect Immun. 1977 Apr;16(1):107–109. doi: 10.1128/iai.16.1.107-109.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakaguchi G., Sakaguchi S., Kondo H. Rapid bioassay for Clostridium botulinum type-E toxins by intravenous injection into mice. Jpn J Med Sci Biol. 1968 Dec;21(6):369–378. doi: 10.7883/yoken1952.21.369. [DOI] [PubMed] [Google Scholar]
- Smart J. L., Roberts T. A. Bovine botulism. Vet Rec. 1977 Sep 10;101(11):201–202. doi: 10.1136/vr.101.11.201. [DOI] [PubMed] [Google Scholar]