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. 1979 Nov;38(5):783–788. doi: 10.1128/aem.38.5.783-788.1979

Coexistence of different pathways in the metabolism of n-propylbenzene by Pseudomonas sp.

Y Jigami, Y Kawasaki, T Omori, Y Minoda
PMCID: PMC243586  PMID: 543699

Abstract

Pseudomonas desmolytica S449B1 and Pseudomonas convexa S107B1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. The same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. A ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. Together with the results of oxygen uptake experiments, the data obtained suggested not only the existence of a reductive step to form 2-hydroxy-6-oxononanoic acid, but also the coexistence of two different pathways in the metabolism of n-propylbenzene by the strains used.

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Selected References

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  1. Baggi G., Catelani D., Galli E., Treccani V. The microbial degradation of phenylalkanes. 2-Phenylbutane, 3-phenylpentane, 3-phenyldodecane and 4-phenylheptane. Biochem J. 1972 Mar;126(5):1091–1097. doi: 10.1042/bj1261091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayly R. C., Dagley S., Gibson D. T. The metabolism of cresols by species of Pseudomonas. Biochem J. 1966 Nov;101(2):293–301. doi: 10.1042/bj1010293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Catelani D., Fiecchi A., Galli E. Formation of 2-hydroxy-6-oxo-2, trans-4, trans-heptad-ienoic acid from 3-methylcatechol by a Pseudomonas. Experientia. 1968 Feb 15;24(2):113–113. doi: 10.1007/BF02146927. [DOI] [PubMed] [Google Scholar]
  4. DAGLEY S., CHAPMAN P. J., GIBSON D. T., WOOD J. M. DEGRADATION OF THE BENZENE NUCLEUS BY BACTERIA. Nature. 1964 May 23;202:775–778. doi: 10.1038/202775a0. [DOI] [PubMed] [Google Scholar]
  5. DAVIS J. B., RAYMOND R. L. Oxidation of alkyl-substituted cyclic hydrocarbons by a Nocardia during growth on n-alkanes. Appl Microbiol. 1961 Sep;9:383–388. doi: 10.1128/am.9.5.383-388.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EL-BAGOURY S., FLETCHER S., MORRISON R. B. Effect of chloramphenicol in maintaining the viability of Escherichia coli. Nature. 1956 Dec 29;178(4548):1467–1467. doi: 10.1038/1781467a0. [DOI] [PubMed] [Google Scholar]
  7. Evans W. C. Oxidation of phenol and benzoic acid by some soil bacteria. Biochem J. 1947;41(3):373–382. doi: 10.1042/bj0410373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gibson D. T., Cardini G. E., Maseles F. C., Kallio R. E. Incorporation of oxygen-18 into benzene by Pseudomonas putida. Biochemistry. 1970 Mar 31;9(7):1631–1635. doi: 10.1021/bi00809a024. [DOI] [PubMed] [Google Scholar]
  9. Gibson D. T., Gschwendt B., Yeh W. K., Kobal V. M. Initial reactions in the oxidation of ethylbenzene by Pseudomonas putida. Biochemistry. 1973 Apr 10;12(8):1520–1528. doi: 10.1021/bi00732a008. [DOI] [PubMed] [Google Scholar]
  10. Jamison V. W., Raymond R. L., Hudson J. O. Microbial Hydrocarbon Co-oxidation. III. Isolation and Characterization of an alpha, alpha'-Dimethyl-cis, cis-Muconic Acid-producing Strain of Nocardia corallina. Appl Microbiol. 1969 Jun;17(6):853–856. doi: 10.1128/am.17.6.853-856.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McKenna E. J., Coon M. J. Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans. J Biol Chem. 1970 Aug 10;245(15):3882–3889. [PubMed] [Google Scholar]
  12. Raymond R. L., Jamison V. W., Hudson J. O. Microbial hydrocarbon co-oxidation. I. Oxidation of mono- and dicyclic hydrocarbons by soil isolates of the genus Nocardia. Appl Microbiol. 1967 Jul;15(4):857–865. doi: 10.1128/am.15.4.857-865.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Raymond R. L., Jamison V. W., Hudson J. O. Microbial hydrocarbon co-oxidation. II. Use of ion-exchange resins. Appl Microbiol. 1969 Apr;17(4):512–515. doi: 10.1128/am.17.4.512-515.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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