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. 1979 Nov;38(5):1011–1014. doi: 10.1128/aem.38.5.1011-1014.1979

Two Improved Methods for Obtaining Axenic Cultures of Cyanobacteria

Timo Vaara 1, Martti Vaara 1, Seppo Niemelä 1
PMCID: PMC243622  PMID: 16345455

Abstract

Scoring the agar plate before incubation under unidirectional light led to a rapid separation of gliding filamentous cyanobacteria from their contaminating bacteria. Twenty strains were purified by the method. Additionally, 13 axenic cyanobacterial strains were isolated from pour plates made after treatment of cyanobacterial cultures in tryptone-yeast extract-glucose broth with cycloserine in darkness to select for obligate photoautotrophs.

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Selected References

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  1. BUNT J. S. Isolation of bacteria-free cultures from hormogone-producing blue-green algae. Nature. 1961 Dec 30;192:1275–1276. doi: 10.1038/1921275a0. [DOI] [PubMed] [Google Scholar]
  2. BURTON S. D., MORITA R. Y. EFFECT OF CATALASE AND CULTURAL CONDITIONS ON GROWTH OF BEGGIATOA. J Bacteriol. 1964 Dec;88:1755–1761. doi: 10.1128/jb.88.6.1755-1761.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bowyer J. W., Skerman V. B. Production of axemic cultures of soil-borne and endophytic blue-green algae. J Gen Microbiol. 1968 Dec;54(2):299–306. doi: 10.1099/00221287-54-2-299. [DOI] [PubMed] [Google Scholar]
  4. Burton S. D., Lee J. D. Improved enrichment and isolation procedures for obtaining pure cultures of beggiatoa. Appl Environ Microbiol. 1978 Mar;35(3):614–617. doi: 10.1128/aem.35.3.614-617.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sitz T. O., Schmidt R. R. Purification of Synechococcus lividus by equilibrium centrifugation and its synchronization by differential centrifugation. J Bacteriol. 1973 Jul;115(1):43–46. doi: 10.1128/jb.115.1.43-46.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Waterbury J. B., Stanier R. Y. Patterns of growth and development in pleurocapsalean cyanobacteria. Microbiol Rev. 1978 Mar;42(1):2–44. doi: 10.1128/mr.42.1.2-44.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wieringa K. T. A new method for obtaining bacteria-free cultures of blue-green algae. Antonie Van Leeuwenhoek. 1968;34(1):54–56. doi: 10.1007/BF02046413. [DOI] [PubMed] [Google Scholar]
  9. ZEHNDER A., HUGHES E. O. The antialgal activity of acti-dione. Can J Microbiol. 1958 Aug;4(4):399–408. doi: 10.1139/m58-042. [DOI] [PubMed] [Google Scholar]

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