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. 1981 Jan;41(1):71–76. doi: 10.1128/aem.41.1.71-76.1981

Production of Skatole and para-Cresol by a Rumen Lactobacillus sp.

Melvin T Yokoyama 1, James R Carlson 2
PMCID: PMC243641  PMID: 16345702

Abstract

The objective of this study was to examine the substrate specificity of several ruminal strains of a Lactobacillus sp. which previously was shown to produce skatole (3-methylindole) by the decarboxylation of indoleacetic acid. A total of 13 compounds were tested for decarboxylase activity. The Lactobacillus strains produced p-cresol (4-methylphenol) by the decarboxylation of p-hydroxyphenylacetic acid, but did not produce either o-cresol or m-cresol from the corresponding hydroxyphenylacetic acid isomers. These strains also decarboxylated 5-hydroxyindoleacetic acid to 5-hydroxyskatole and 3,4-dihydroxyphenylacetic acid to methylcatechol. Skatole and p-cresol were produced in a 0.5:1 ratio, when indoleacetic acid and p-hydroxyphenylacetic acid were combined in equimolar concentrations. Competition studies with indoleacetic acid and p-hydroxyphenylacetic acid suggested that two different decarboxylating enzymes are involved in the production of skatole and p-cresol by these strains. This is the first demonstration of both skatole production and p-cresol production by a single bacterium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRYANT M. P., ROBINSON I. M. Some nutritional characteristics of predominant culturable ruminal bacteria. J Bacteriol. 1962 Oct;84:605–614. doi: 10.1128/jb.84.4.605-614.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bakke O. M. Degradation of DOPA by intestinal microorganisms in vitro. Acta Pharmacol Toxicol (Copenh) 1971;30(1):115–121. doi: 10.1111/j.1600-0773.1971.tb00640.x. [DOI] [PubMed] [Google Scholar]
  3. Bakke O. M. Studies on the degradation of tyrosine by rat caecal contents. Scand J Gastroenterol. 1969;4(7):603–608. [PubMed] [Google Scholar]
  4. Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
  5. Caldwell D. R., Bryant M. P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol. 1966 Sep;14(5):794–801. doi: 10.1128/am.14.5.794-801.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Calne D. B., Karoum F., Ruthven C. R., Sandler M. The metabolism of orally administered L-Dopa in Parkinsonism. Br J Pharmacol. 1969 Sep;37(1):57–68. doi: 10.1111/j.1476-5381.1969.tb09522.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carlson J. R., Dickinson E. O., Yokoyama M. T., Bradley B. Pulmonary edema and emphysema in cattle after intraruminal and intravenous administration of 3-methylindole. Am J Vet Res. 1975 Sep;36(9):1341–1347. [PubMed] [Google Scholar]
  8. Carlson J. R., Yokoyama M. T., Dickinson E. O. Induction of pulmonary edema and emphysema in cattle and goats with 3-methylindole. Science. 1972 Apr 21;176(4032):298–299. doi: 10.1126/science.176.4032.298. [DOI] [PubMed] [Google Scholar]
  9. Elsden S. R., Hilton M. G., Waller J. M. The end products of the metabolism of aromatic amino acids by Clostridia. Arch Microbiol. 1976 Apr 1;107(3):283–288. doi: 10.1007/BF00425340. [DOI] [PubMed] [Google Scholar]
  10. Fellers C. R., Clough R. W. INDOL AND SKATOL DETERMINATION IN BACTERIAL CULTURES. J Bacteriol. 1925 Mar;10(2):105–133. doi: 10.1128/jb.10.2.105-133.1925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mori A., Yasaka Y., Masamoto K., Hiramatsu M. Gas chromatography of 5-hydroxy-3-methylindole in human urine. Clin Chim Acta. 1978 Mar 1;84(1-2):63–68. doi: 10.1016/0009-8981(78)90477-1. [DOI] [PubMed] [Google Scholar]
  13. Scheline R. R. Metabolism of phenolic acids by the rat intestinal microflora. Acta Pharmacol Toxicol (Copenh) 1968;26(2):189–205. doi: 10.1111/j.1600-0773.1968.tb00437.x. [DOI] [PubMed] [Google Scholar]
  14. Scott T. W., Ward P. F., Dawson R. M. The formation and metabolism of phenyl-substituted fatty acids in the ruminant. Biochem J. 1964 Jan;90(1):12–24. doi: 10.1042/bj0900012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yokoyama M. T., Carlson J. R., Dickinson E. O. Ruminal and plasma concentrations of 3-methylindole associated with tryptophan-induced pulmonary edema and emphysema in cattle. Am J Vet Res. 1975 Sep;36(9):1349–1352. [PubMed] [Google Scholar]
  16. Yokoyama M. T., Carlson J. R. Dissimilation of tryptophan and related indolic compounds by ruminal microorganisms in vitro. Appl Microbiol. 1974 Mar;27(3):540–548. doi: 10.1128/am.27.3.540-548.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yokoyama M. T., Carlson J. R., Holdeman L. V. Isolation and characteristics of a skatole-producing Lactobacillus sp. from the bovine rumen. Appl Environ Microbiol. 1977 Dec;34(6):837–842. doi: 10.1128/aem.34.6.837-842.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yokoyama M. T., Carlson J. R. Microbial metabolites of tryptophan in the intestinal tract with special reference to skatole. Am J Clin Nutr. 1979 Jan;32(1):173–178. doi: 10.1093/ajcn/32.1.173. [DOI] [PubMed] [Google Scholar]

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