Abstract
Uptake of poliovirus 1 by the blue crab, Callinectes sapidus, was measured to assess the likelihood of contamination by human enteric viruses. Virus was found in all parts of the crab within 2 h after the crab was placed in contaminated artificial seawater. The highest concentrations of virus were found in the hemolymph and digestive tract, but the meat also contained virus. The concentration of virus in the crabs was generally less than in the surrounding water. Changes in salinity did not substantially affect the rate of accumulation. An increase in temperature from 15 to 25 degrees C increased the rates of both uptake and removal. Poliovirus survived up to 6 days in crabs at a temperature of 15 degrees C and a salinity of 10 g/kg. When contaminated crabs were boiled, 99.9% of poliovirus 1, simian rotavirus SA11, and a natural isolate of echovirus 1 were inactivated within 8 min. These data demonstrate that viruses in crabs should not pose a serious health hazard if recommended cooking procedures are used.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Canzonier W. J. Accumulation and elimination of coliphage S-13 by the hard clam, Mercenaria mercenaria. Appl Microbiol. 1971 Jun;21(6):1024–1031. doi: 10.1128/am.21.6.1024-1031.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiGirolamo R., Liston J., Matches J. R. Survival of virus in chilled, frozen, and processed oysters. Appl Microbiol. 1970 Jul;20(1):58–63. doi: 10.1128/am.20.1.58-63.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiGirolamo R., Wiczynski L., Daley M., Miranda F. Preliminary observations on the uptake poliovirus by West Coast shore crabs. Appl Microbiol. 1972 Jan;23(1):170–171. doi: 10.1128/am.23.1.170-171.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiGirolamo R., Wiczynski L., Daley M., Miranda F., Viehweger C. Uptake of bacteriophage and their subsequent survival in edible West Coast crabs after processing. Appl Microbiol. 1972 Jun;23(6):1073–1076. doi: 10.1128/am.23.6.1073-1076.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamblet F. E., Hill W. F., Jr, Akin E. W., Benton W. H. Oysters and human viruses: effect of seawater turbidity on poliovirus uptake and elimination. Am J Epidemiol. 1969 May;89(5):562–571. doi: 10.1093/oxfordjournals.aje.a120969. [DOI] [PubMed] [Google Scholar]
- Hoff J. C., Becker R. C. The accumulation and elimination of crude and clarified poliovirus suxpensions by shellfish. Am J Epidemiol. 1969 Jul;90(1):53–61. doi: 10.1093/oxfordjournals.aje.a121049. [DOI] [PubMed] [Google Scholar]
- Larkin E. P., Fassolitis A. C. Viral heat resistance and infectious ribonucleic acid. Appl Environ Microbiol. 1979 Oct;38(4):650–655. doi: 10.1128/aem.38.4.650-655.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liew P. F., Gerba C. P. Thermostabilization of enteroviruses by estuarine sediment. Appl Environ Microbiol. 1980 Aug;40(2):305–308. doi: 10.1128/aem.40.2.305-308.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu O. C., Seraichekas H. R., Murphy B. L. Viral depuration of the Northern quahaug. Appl Microbiol. 1967 Mar;15(2):307–315. doi: 10.1128/am.15.2.307-315.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu O. C., Seraichekas H. R., Murphy B. L. Viral pollution of shellfish. 1. Some basic facts of uptake. Proc Soc Exp Biol Med. 1966 Nov;123(2):481–487. doi: 10.3181/00379727-123-31520. [DOI] [PubMed] [Google Scholar]
- Mitchell J. R., Presnell M. W., Akin E. W., Cummins J. M., Liu O. C. Accumulation and elimination of poliovirus by the eastern oyster. Am J Epidemiol. 1966 Jul;84(1):40–50. doi: 10.1093/oxfordjournals.aje.a120626. [DOI] [PubMed] [Google Scholar]
- Smith E. M., Estes M. K., Graham D. Y., Gerba C. P. A plaque assay for the simian rotavirus SAII. J Gen Virol. 1979 Jun;43(3):513–519. doi: 10.1099/0022-1317-43-3-513. [DOI] [PubMed] [Google Scholar]