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. 1981 Jan;41(1):255–260. doi: 10.1128/aem.41.1.255-260.1981

Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.

M C Croxson, A R Bellamy
PMCID: PMC243673  PMID: 6261684

Abstract

A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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