Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1981 Feb;41(2):366–370. doi: 10.1128/aem.41.2.366-370.1981

Growth Inhibition of Streptomyces Species by l-Serine and Its Effect on Tetracycline Biosynthesis

Jose L Parada 1,†
PMCID: PMC243700  PMID: 16345709

Abstract

The addition of serine to minimal medium inhibited the growth of Streptomyces aureofaciens and Streptomyces rimosus. Both the outgrowth of spores and the growth of vegetative cells were inhibited by l-serine. This effect was independent of the carbon source used. In rich nutrient medium, however, the serine effect was not observed. The presence of glycine and methionine in minimal medium reversed the growth inhibition imposed by serine, suggesting that a metabolic block related to the synthesis of these two amino acids was involved. A serine-tolerant mutant of S. aureofaciens isolated after ultraviolet irradiation showed a level of serine deaminase comparable to that of the wild-type strain, which indicated that tolerance to serine was not due to the presence of a more active deaminating enzyme in the mutant. Serine markedly reduced tetracycline and oxytetracycline biosynthesis with the parental strains of Streptomyces spp. The serine-tolerant mutant, however, produced almost the same amount of tetracycline in the presence or absence of serine. The final cell population in fermentation broth was not significantly reduced by l-serine, and the addition of glycine and methionine did not increase the tetracycline yields, which suggested that l-serine inhibition of antibiotic biosynthesis was by a mechanism different from that related to growth inhibition.

Full text

PDF
366

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABELSON P. H. Amino acid biosynthesis in Escherichia coli: isotopic competition with C14-glucose. J Biol Chem. 1954 Jan;206(1):335–343. [PubMed] [Google Scholar]
  2. COHEN G., JACOB F. Sur la répression de la synthèse des enzymes intervenant dans la formation du tryptophane chez Escherichia coll. C R Hebd Seances Acad Sci. 1959 Jun 15;248(24):3490–3492. [PubMed] [Google Scholar]
  3. Cosloy S. D., McFall E. L-Serine-sensitive mutants of Escherichia coli K-12. J Bacteriol. 1970 Sep;103(3):840–841. doi: 10.1128/jb.103.3.840-841.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HENDLIN D., DULANEY E. L., DRESCHER D., COOK T., CHAIET L. Methionine dependence and the biosynthesis of 6-demethylchlortetracycline. Biochim Biophys Acta. 1962 Apr 23;58:635–636. doi: 10.1016/0006-3002(62)90090-2. [DOI] [PubMed] [Google Scholar]
  5. Ingram L. O., Jensen R. A. Growth inhibition by L-phenylalanine in Agmenellum quadruplicatum. A clue to some amino acid interrelationships. Arch Mikrobiol. 1973 Jun 6;91(3):221–233. doi: 10.1007/BF00408909. [DOI] [PubMed] [Google Scholar]
  6. Isenberg S., Newman E. B. Studies on L-serine deaminase in Escherichia coli K-12. J Bacteriol. 1974 Apr;118(1):53–58. doi: 10.1128/jb.118.1.53-58.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson C. L., Vishniac W. Growth Inhibition in Thiobacillus neapolitanus by Histidine, Methionine, Phenylalanine, and Threonine. J Bacteriol. 1970 Dec;104(3):1145–1150. doi: 10.1128/jb.104.3.1145-1150.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEAVITT R. I., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. XI. Valine inhibition of the growth of Escherichia coli strain K-12. J Bacteriol. 1962 Mar;83:624–630. doi: 10.1128/jb.83.3.624-630.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MILLER P. A., MCCORMICK J. R., DOERSCHUK A. P. Studies of chlorotetracycline biosynthesis and the preparation of chlorotetracycline-C14. Science. 1956 Jun 8;123(3206):1030–1031. doi: 10.1126/science.123.3206.1030. [DOI] [PubMed] [Google Scholar]
  10. MONOD J., CHANGEUX J. P., JACOB F. Allosteric proteins and cellular control systems. J Mol Biol. 1963 Apr;6:306–329. doi: 10.1016/s0022-2836(63)80091-1. [DOI] [PubMed] [Google Scholar]
  11. PARDEE A. B., PRESTIDGE L. S. Induced formation of serine and threonine deaminases by Escherichia coli. J Bacteriol. 1955 Dec;70(6):667–674. doi: 10.1128/jb.70.6.667-674.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Parada J. L. Selective filtration in the isolation of independent clones of Streptomyces. Appl Microbiol. 1975 Jan;29(1):13–16. doi: 10.1128/am.29.1.13-16.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RILEY M., PARDEE A. B. Gene expression: its specificity and regulation. Annu Rev Microbiol. 1962;16:1–34. doi: 10.1146/annurev.mi.16.100162.000245. [DOI] [PubMed] [Google Scholar]
  14. Stadtman E. R. Allosteric regulation of enzyme activity. Adv Enzymol Relat Areas Mol Biol. 1966;28:41–154. doi: 10.1002/9780470122730.ch2. [DOI] [PubMed] [Google Scholar]
  15. Stauffer G. V., Baker C. A., Brenchley J. E. Regulation of serine transhydroxymethylase activity in Salmonella typhimurium. J Bacteriol. 1974 Dec;120(3):1017–1025. doi: 10.1128/jb.120.3.1017-1025.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stauffer G. V., Brenchley J. E. Influence of methionine biosynthesis on serine transhydroxymethylase regulation in Salmonella typhimurium LT2. J Bacteriol. 1977 Feb;129(2):740–749. doi: 10.1128/jb.129.2.740-749.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Umbarger H. E. Regulation of amino acid metabolism. Annu Rev Biochem. 1969;38:323–370. doi: 10.1146/annurev.bi.38.070169.001543. [DOI] [PubMed] [Google Scholar]
  18. VIRGILIO A., HENGELLER C. [Production of tetracycline by Streptomyces psammoticus]. Farmaco Sci. 1960 Mar;15:164–174. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES