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. 1981 Feb;41(2):455–458. doi: 10.1128/aem.41.2.455-458.1981

Urea-lysine method for recovery of enteroviruses from sludge.

S R Farrah, P R Scheuerman, G Bitton
PMCID: PMC243715  PMID: 6263183

Abstract

Enteroviruses added to sludge and indigenous viruses present in sludge were recovered by treating the sludge flocs with a 4 M urea solution buffered at pH 9 with 0.5 M lysine. Eluted viruses were absorbed to aluminum hydroxide flocs and collected by centrifugation. The flocs were solubilized with 0.1 M ethylenediaminetetraacetic acid-3% beef extract at pH 9. After dialysis to remove the ethylenediaminetraacetic acid, viruses were further concentrated by organic flocculation. Approximately 40% of poliovirus and coxsackievirus B-3 added to 500 to 1,000 ml of sludge could be recovered in final sample volumes of less than 10 ml. Polioviruses, echoviruses, and coxsackieviruses were recovered from different samples of wastewater sludge.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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