Abstract
Anaerobic and facultatively anaerobic bacteria associated with soft rot of yam (Diascorea rotundata) were isolated by the looping-out method and found to consist of Clostridium (three isolates), Corynebacterium (three isolates), Vibrio (one isolate), and Bacillus lentus (one isolate). Enzyme assay for hydrolase, lyase, and pectinesterase activities by the cup-plate method showed that except for Vibrio sp., B. lentus, and two isolates of Corynebacterium no pectinase activity could be detected for organisms cultured on pectin medium. Most of the cultures on yam tissue, however, showed activities for the three enzymes. The viscometric assay for hydrolase and lyase enzymes indicated a significant level of hydrolase activity (a 40.90% decrease in viscosity for Vibrio sp. and Corynebacterium spp.), but no lyase activity for most of the isolates. Two isolates of Corynebacterium and B. lentus caused changes in fresh yams suggestive of soft rot.
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- Bateman D. F. Hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes of Fusarium solani f. phaseoli. Phytopathology. 1966 Feb;56(2):238–244. [PubMed] [Google Scholar]
- Codner R. C. Symposium on microbial changes in foods. Pectinolytic and cellulolytic enzymes in the microbial modification of plant tissues. J Appl Bacteriol. 1971 Mar;34(1):147–160. doi: 10.1111/j.1365-2672.1971.tb02274.x. [DOI] [PubMed] [Google Scholar]
- Fogarty W. M., Ward O. P. Pectinases and pectic polysaccharides. Prog Ind Microbiol. 1974;13:61–119. [PubMed] [Google Scholar]
- HELLINGER E. Sporulating anaerobes on English flax. Nature. 1953 Jun 20;171(4364):1119–1119. doi: 10.1038/1711119a0. [DOI] [PubMed] [Google Scholar]
- Lund B. M. Isolation of pectolytic clostridia from potatoes. J Appl Bacteriol. 1972 Dec;35(4):609–614. doi: 10.1111/j.1365-2672.1972.tb03742.x. [DOI] [PubMed] [Google Scholar]
- McCOMB E. A., McCREADY R. M. Use of the hydroxamic acid reaction for determining pectinesterase activity. Stain Technol. 1958 May;33(3):129–131. doi: 10.3109/10520295809111836. [DOI] [PubMed] [Google Scholar]
- Moran F., Nasuno S., Starr M. P. Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora. Arch Biochem Biophys. 1968 Feb;123(2):298–306. doi: 10.1016/0003-9861(68)90138-0. [DOI] [PubMed] [Google Scholar]
- Nasuno S., Starr M. P. Polygalacturonase of Erwinia carotovora. J Biol Chem. 1966 Nov 25;241(22):5298–5306. [PubMed] [Google Scholar]