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. 1981 May;41(5):1214–1218. doi: 10.1128/aem.41.5.1214-1218.1981

Fermentative Conversion of Cellulose to Acetic Acid and Cellulolytic Enzyme Production by a Bacterial Mixed Culture Obtained from Sewage Sludge †

A W Khan 1, Duncan Wall 1, L van den Berg 1
PMCID: PMC243891  PMID: 16345772

Abstract

A simple procedure that uses a cellulose-enriched culture started from sewage sludge was developed for producing cellulolytic enzymes and converting cellulose to acetic acid rather than CH4 and CO2. In this procedure, the culture which converts cellulose to CH4 and CO2 was mixed with a synthetic medium and cellulose and heated to 80°C for 15 min before incubation. The end products formed were acetic acid, propionic acid, CO2, and traces of ethanol and H2. Supernatants from 6- to 10-day-old cultures contained 16 to 36 mM acetic acid. Cellulolytic enzymes in the supernatant were stable at 2°C under aerobic conditions for up to 4 weeks and had the ability to hydrolyze carboxymethyl cellulose, a microcystalline cellulose, cellobiose, xylan, and filter paper to reducing sugars.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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