Abstract
Nearly 10(6) cells of Cryptococcus neoformans were cultured per g of pigeon droppings in a vacant tower. The air in the tower contained an average of 45 viable cells of C. neoformans per 100 liters: 60% of the cells were less than 4.7 micron in diameter. It is estimated that a human exposed to this atmosphere for 1 h would have 41 cells of c. neoformans deposited in the lungs. Sweeping resulted in the aerosolization of large numbers of cells of C. neoformans from 4.7 to 11 micron in diameter, the number of cells less than 4.7 micron remained relatively constant. One minute after sweeping, 4.4% of viable airborne cells of C. neoformans were less than 1.1 micron in diameter. We believe that this is the first report of isolating such small cells of C. neoformans from a natural site.
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Selected References
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- ANDERSEN A. A. New sampler for the collection, sizing, and enumeration of viable airborne particles. J Bacteriol. 1958 Nov;76(5):471–484. doi: 10.1128/jb.76.5.471-484.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown J. H., Cook K. M., Ney F. G., Hatch T. Influence of Particle Size upon the Retention of Particulate Matter in the Human Lung. Am J Public Health Nations Health. 1950 Apr;40(4):450–480. doi: 10.2105/ajph.40.4.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farhi F., Bulmer G. S., Tacker J. R. Cryptococcus neoformans IV. The Not-So-Encapsulated Yeast. Infect Immun. 1970 Jun;1(6):526–531. doi: 10.1128/iai.1.6.526-531.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green J. R., Bulmer G. S. Gastrointestinal inoculation of Cryptococcus neoformans in mice. Sabouraudia. 1979 Sep;17(3):233–240. [PubMed] [Google Scholar]
- HATCH T. F. Distribution and deposition of inhaled particles in respiratory tract. Bacteriol Rev. 1961 Sep;25:237–240. doi: 10.1128/br.25.3.237-240.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishaq C. M., Bulmer G. S., Felton F. G. An evaluation of various environmental factors affecting the propagation of Cryptococcus neoformas. Mycopathol Mycol Appl. 1968 Jul 12;35(2):81–90. doi: 10.1007/BF02049570. [DOI] [PubMed] [Google Scholar]
- Neilson J. B., Fromtling R. A., Bulmer G. S. Cryptococcus neoformans: size range of infectious particles from aerosolized soil. Infect Immun. 1977 Sep;17(3):634–638. doi: 10.1128/iai.17.3.634-638.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Powell K. E., Dahl B. A., Weeks R. J., Tosh F. E. Airborne Cryptococcus neoformans: particles from pigeon excreta compatible with alveolar deposition. J Infect Dis. 1972 Apr;125(4):412–415. doi: 10.1093/infdis/125.4.412. [DOI] [PubMed] [Google Scholar]
- Ruiz A., Fromtling R. A., Bulmer G. S. Distribution of Cryptococcus neoformans in a natural site. Infect Immun. 1981 Feb;31(2):560–563. doi: 10.1128/iai.31.2.560-563.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shields A. B., Ajello L. Medium for selective isolation of Cryptococcus neoformans. Science. 1966 Jan 14;151(3707):208–209. doi: 10.1126/science.151.3707.208. [DOI] [PubMed] [Google Scholar]
- Staib F., Seeliger H. P. Un nouveau milieu sélectif pour l'isolement de C. neoformans des matières fécales et du sol. Ann Inst Pasteur (Paris) 1966 May;110(5):792–793. [PubMed] [Google Scholar]
- TAKOS M. J. Experimental cryptococcosis produced by the ingestion of virulent organisms. N Engl J Med. 1956 Mar 29;254(13):598–601. doi: 10.1056/NEJM195603292541303. [DOI] [PubMed] [Google Scholar]
- Vickers R. M., McElligott J. J., Jr, Rihs J. D., Postic B. Medium containing trypan blue and antibiotics for the detection of Cryptococcus neoformans in clinical samples. Appl Microbiol. 1974 Jan;27(1):38–42. doi: 10.1128/am.27.1.38-42.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]