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. 1981 Jun;41(6):1363–1373. doi: 10.1128/aem.41.6.1363-1373.1981

Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO2 to Methanogenesis in Cattle Waste at 40 and 60°C

Roderick I Mackie 1,†, Marvin P Bryant 1
PMCID: PMC243924  PMID: 16345789

Abstract

The quantitative contribution of fatty acids and CO2 to methanogenesis was studied by using stirred, 3-liter bench-top digestors fed on a semicontinuous basis with cattle waste. The fermentations were carried out at 40 and 60°C under identical loading conditions (6 g of volatile solids per liter of reactor volume per day, 10-day retention time). In the thermophilic digestor, acetate turnover increased from a prefeeding level of 16 μM/min to a peak (49 μM/min) 1 h after feeding and then gradually decreased. Acetate turnover in the mesophilic digestor increased from 15 to 40 μM/min. Propionate turnover ranged from 2 to 5.2 and 1.5 to 4.5 μM/min in the thermophilic and mesophilic digestors, respectively. Butyrate turnover (0.7 to 1.2 μM/min) was similar in both digestors. The proportion of CH4 produced via the methyl group of acetate varied with time after feeding and ranged from 72 to 75% in the mesophilic digestor and 75 to 86% in the thermophilic digestor. The contribution from CO2 reduction was 24 to 29% and 19 to 27%, respectively. Propionate and butyrate turnover accounted for 20% of the total CH4 produced. Acetate synthesis from CO2 was greatest shortly after feeding and was higher in the thermophilic digestor (0.5 to 2.4 μM/min) than the mesophilic digestor (0.3 to 0.5 μM/min). Counts of fatty acid-degrading bacteria were related to their turnover activity.

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Selected References

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  1. Andreesen J. R., Schaupp A., Neurauter C., Brown A., Ljungdahl L. G. Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: effect of metals on growth yield, enzymes, and the synthesis of acetate from CO 2 . J Bacteriol. 1973 May;114(2):743–751. doi: 10.1128/jb.114.2.743-751.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boone D. R., Bryant M. P. Propionate-Degrading Bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from Methanogenic Ecosystems. Appl Environ Microbiol. 1980 Sep;40(3):626–632. doi: 10.1128/aem.40.3.626-632.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
  5. CHANEY A. L., MARBACH E. P. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed] [Google Scholar]
  6. Cappenberg T. E., Prins R. A. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. 3. Experiments with 14C-labeled substrates. Antonie Van Leeuwenhoek. 1974;40(3):457–469. doi: 10.1007/BF00399358. [DOI] [PubMed] [Google Scholar]
  7. Iannotti E. L., Fischer J. R., Sievers D. M. Medium for the enumeration and isolation of bacteria from a Swine waste digester. Appl Environ Microbiol. 1978 Oct;36(4):555–566. doi: 10.1128/aem.36.4.555-566.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaspar H. F., Wuhrmann K. Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge. Appl Environ Microbiol. 1978 Jul;36(1):1–7. doi: 10.1128/aem.36.1.1-7.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McInerney M. J., Bryant M. P., Hespell R. B., Costerton J. W. Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium. Appl Environ Microbiol. 1981 Apr;41(4):1029–1039. doi: 10.1128/aem.41.4.1029-1039.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mountfort D. O., Asher R. A. Changes in proportions of acetate and carbon dioxide used as methane precursors during the anaerobic digestion of bovine waste. Appl Environ Microbiol. 1978 Apr;35(4):648–654. doi: 10.1128/aem.35.4.648-654.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Brien W. E., Ljungdahl L. G. Fermentation of fructose and synthesis of acetate from carbon dioxide by Clostridium formicoaceticum. J Bacteriol. 1972 Feb;109(2):626–632. doi: 10.1128/jb.109.2.626-632.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ohwaki K., Hungate R. E. Hydrogen utilization by clostridia in sewage sludge. Appl Environ Microbiol. 1977 Jun;33(6):1270–1274. doi: 10.1128/aem.33.6.1270-1274.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pfennig N., Biebl H. Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium. Arch Microbiol. 1976 Oct 11;110(1):3–12. doi: 10.1007/BF00416962. [DOI] [PubMed] [Google Scholar]
  14. Salanitro J. P., Muirhead P. A. Quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. Appl Microbiol. 1975 Mar;29(3):374–381. doi: 10.1128/am.29.3.374-381.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith P. H., Mah R. A. Kinetics of acetate metabolism during sludge digestion. Appl Microbiol. 1966 May;14(3):368–371. doi: 10.1128/am.14.3.368-371.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Strayer R. F., Tiedje J. M. Kinetic parameters of the conversion of methane precursors to methane in a hypereutrophic lake sediment. Appl Environ Microbiol. 1978 Aug;36(2):330–340. doi: 10.1128/aem.36.2.330-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Der Walt J. G. The separation of some volatile fatty acids on a "Sephadex" partition chromatogram. Onderstepoort J Vet Res. 1977 Jun;44(2):69–72. [PubMed] [Google Scholar]
  18. Varel V. H., Isaacson H. R., Bryant M. P. Thermophilic methane production from cattle waste. Appl Environ Microbiol. 1977 Feb;33(2):298–307. doi: 10.1128/aem.33.2.298-307.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weimer P. J., Zeikus J. G. Acetate metabolism in Methanosarcina barkeri. Arch Microbiol. 1978 Nov 13;119(2):175–182. doi: 10.1007/BF00964270. [DOI] [PubMed] [Google Scholar]
  20. Widdel F., Pfennig N. A new anaerobic, sporing, acetate-oxidizing, sulfate-reducing bacterium, Desulfotomaculum (emend.) acetoxidans. Arch Microbiol. 1977 Feb 4;112(1):119–122. doi: 10.1007/BF00446665. [DOI] [PubMed] [Google Scholar]
  21. Winfrey M. R., Zeikus J. G. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl Environ Microbiol. 1977 Feb;33(2):275–281. doi: 10.1128/aem.33.2.275-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zehnder A. J., Brock T. D. Anaerobic methane oxidation: occurrence and ecology. Appl Environ Microbiol. 1980 Jan;39(1):194–204. doi: 10.1128/aem.39.1.194-204.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zehnder A. J., Brock T. D. Methane formation and methane oxidation by methanogenic bacteria. J Bacteriol. 1979 Jan;137(1):420–432. doi: 10.1128/jb.137.1.420-432.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zehnder A. J., Huser B. A., Brock T. D., Wuhrmann K. Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol. 1980 Jan;124(1):1–11. doi: 10.1007/BF00407022. [DOI] [PubMed] [Google Scholar]
  25. Zehnder A. J., Huser B., Brock T. D. Measuring radioactive methane with the liquid scintillation counter. Appl Environ Microbiol. 1979 May;37(5):897–899. doi: 10.1128/aem.37.5.897-899.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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