Abstract
A strain of Stachybotrys atra isolated from a field case of stachybotryotoxicosis in Hungary was cultured in Hungary. All of the compounds toxic to brine shrimp were separated from the culture extract by solvent partition, column chromatography, and preparative thin-layer chromatography. Two of the toxic compounds were identified as verrucarin J and satratoxin H by comparison with pure standards resolved by high-pressure liquid chromatography and characterized by mass spectrometry. Two other toxic components were identified as roriden E and satratoxin G on the basis of their mass spectra. The fifth toxic compound was identified as a macrocyclic trichothecene based on the following findings: a positive 4-(p-nitrobenzyl)pyridine color reaction, hydrolysis resulting in verrucarol verified by combined gas chromatography-mass spectrometry, and a characteristic trichothecene proton-nuclear magnetic resonance spectrum. This macrocyclic trichothecene has a molecular ion (528) identical to satratoxin H, and its mass spectrum is similar; however, its Rf value on Silica Gel G differs.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hayakawa S., Kondo E., Wakisaka Y., Minato H., Katagiri K. Vertisporin, a new antibiotic from Verticimonosporium diffractum. J Antibiot (Tokyo) 1975 Jul;28(7):550–551. doi: 10.7164/antibiotics.28.550. [DOI] [PubMed] [Google Scholar]
- Jarvis B. B., Stahly G. P., Pavanasasivam G., Mazzola E. P. Structure of roridin J, a new macrocyclic trichothecene from Myrothecium verrucaria. J Antibiot (Tokyo) 1980 Feb;33(2):256–258. doi: 10.7164/antibiotics.33.256. [DOI] [PubMed] [Google Scholar]
- Korpinen E. L., Kurkinen M., Nummi M., Enari T. M. Studies on Stachybotrys alternans. 3. Chromatographic separation and tissue culture toxicity test of Stachybotrys toxins. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Feb;82(1):7–11. [PubMed] [Google Scholar]
- Le Bars J., Gérard J. P., Michel C. Mise en évidence de la stachybotryotoxicose en France: un cas d'intoxication aiguë chez des daims. Ann Nutr Aliment. 1977;31(4-6):509–517. [PubMed] [Google Scholar]
- Matsumoto M., Minato H., Uotani N., Matsumoto K., Kondo E. New antibiotics from Cylindrocarpon sp. J Antibiot (Tokyo) 1977 Aug;30(8):681–682. doi: 10.7164/antibiotics.30.681. [DOI] [PubMed] [Google Scholar]
- Nummi N., Niku-Paavola M. L. Water soluble toxins of Stachybotrys alternans. Ann Nutr Aliment. 1977;31(4-6):761–770. [PubMed] [Google Scholar]
- Palyusik M. Biological test for the toxic substance of Stachybotrys alternans. Acta Vet Acad Sci Hung. 1970;20(1):57–67. [PubMed] [Google Scholar]
- Schneider D. J., Marasas W. F., Dale Kuys J. C., Kriek N. P., Van Schalkwyk G. C. A field outbreak of suspected stachybotryotoxicosis in sheep. J S Afr Vet Assoc. 1979 Jun;50(2):73–81. [PubMed] [Google Scholar]
- Szathmary C. I., Mirocha C. J., Palyusik M., Pathre S. V. Identification of mycotoxins produced by species of Fusarium and Stachybotrys obtained from Eastern Europe. Appl Environ Microbiol. 1976 Oct;32(4):579–584. doi: 10.1128/aem.32.4.579-584.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takitani S., Asabe Y., Kato T., Suzuki M., Ueno Y. Spectrodensitometric determination of trichothecene mycotoxins with 4-(p-nitrobenzyl)pyridine on silica gel thin-layer chromatograms. J Chromatogr. 1979 Apr 21;172:335–342. doi: 10.1016/s0021-9673(00)90970-1. [DOI] [PubMed] [Google Scholar]
- Traxler P., Zürcher W., Tamm C. Die Struktur des Antibioticums Roridin E. Helv Chim Acta. 1970;53(8):2071–2085. doi: 10.1002/hlca.19700530818. [DOI] [PubMed] [Google Scholar]