Abstract
MacConkey agar, standard M-FC agar, M-FC agar without rosolic acid, M-FC agar with a resuscitation top layer, Teepol agar, and pads saturated with Teepol broth, were evaluated as growth media for membrane filtration counting of fecal coliform bacteria in water. In comparative tests on 312 samples of water from a wide variety of sources, including chlorinated effluents, M-FC agar without rosolic acid proved the medium of choice because it generally yielded the highest counts, was readily obtainable, easy to prepare and handle, and yielded clearly recognizable fecal coliform colonies. Identification of 1,139 fecal coliform isolates showed that fecal coliform tests cannot be used to enumerate Escherichia coli because the incidence of E. coli among fecal coliforms varied from an average of 51% for river water to 93% for an activated sludge effluent after chlorination. The incidence of Klebsiella pneumoniae among fecal coliforms varied from an average of 4% for the activated sludge effluent after chlorination to 32% for the river water. The advantages of a standard membrane filtration procedure for routine counting of fecal coliforms in water using M-FC agar without rosolic acid as growth medium, in the absence of preincubation or resuscitation steps, are outlined.
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Selected References
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- Bagley S. T., Seidler R. J. Significance of fecal coliform-positive Klebsiella. Appl Environ Microbiol. 1977 May;33(5):1141–1148. doi: 10.1128/aem.33.5.1141-1148.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grabow W. O., du Preez M. Comparison of m-Endo LES, MacConkey, and Teepol media for membrane filtration counting of total coliform bacteria in water. Appl Environ Microbiol. 1979 Sep;38(3):351–358. doi: 10.1128/aem.38.3.351-358.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haley C. E., Gunn R. A., Hughes J. M., Lippy E. C., Craun G. F. From the Center for Disease Control. Outbreaks of waterborne diseases in the United States, 1978. J Infect Dis. 1980 Jun;141(6):794–797. doi: 10.1093/infdis/141.6.794. [DOI] [PubMed] [Google Scholar]
- Hayek L. J., Willis G. W. A comparison of two commerical methods for the identification of the Enterobacteriaceae--API 20E and the Enterotube--with conventional methods. J Clin Pathol. 1976 Feb;29(2):158–161. doi: 10.1136/jcp.29.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Livingstone D. J. An appraisal of sewage pollution along a section of the Natal coast. J Hyg (Lond) 1969 Jun;67(2):209–223. doi: 10.1017/s0022172400041619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Presswood W. G., Strong D. K. Modification of M-FC medium by eliminating rosolic acid. Appl Environ Microbiol. 1978 Jul;36(1):90–94. doi: 10.1128/aem.36.1.90-94.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson E. A., MacLowry J. D. Mathematical analysis of the API enteric 20 profile register using a computer diagnostic model. Appl Microbiol. 1974 Oct;28(4):691–695. doi: 10.1128/am.28.4.691-695.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rose R. E., Geldreich E. E., Litsky W. Improved membrane filter method for fecal coliform analysis. Appl Microbiol. 1975 Apr;29(4):532–536. doi: 10.1128/am.29.4.532-536.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tobin R. S., Dutka B. J. Comparison of the surface structure, metal binding, and fecal coliform recoveries of nine membrane filters. Appl Environ Microbiol. 1977 Jul;34(1):69–79. doi: 10.1128/aem.34.1.69-79.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]