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. 1981 Aug;42(2):204–210. doi: 10.1128/aem.42.2.204-210.1981

R factors in coliform-fecal coliform sewage flora of the prairies and Northwest Territories of Canada.

J B Bell, W R Macrae, G E Elliott
PMCID: PMC243991  PMID: 7283425

Abstract

Coliform and fecal coliform populations found in the raw sewages and final sewage effluents of the prairie provinces and the Northwest Territories were examined for antibiotic resistance and the possession of R factors. It was determined that 8.91% of the total coliform and 10.80% of the fecal coliform populations carried R factors. The following numbers of combinations of R determinants were found: 39 in the Escherichia coli population, 6 in the Citrobacter population, 20 in the Enterobacter populations, 10 in the Klebsiella populations, and 11 in the Aeromonas populations. The maximum number of R determinants transferable simultaneously was seven; organisms with R factors containing determinants for chloramphenicol usually contained determinants for ampicillin. Of the coliform and fecal coliform populations, 2 to 4% were resistant to chloramphenicol in some provinces, and from 17 to 30% of the populations were resistant to three or more antibiotics. It was calculated that coliforms containing R factors in the raw sewage reached population levels of 1.5 X 10(7)/100 ml, and fecal coliforms containing R factors reached population levels of 8.6 X 10(5) ml. Final effluent discharges to the receiving environment contained R factor-containing coliform and fecal coliform populations of 3.1 X 10(4)/100 ml and 5.8 X 10(2)/100 ml, respectively. The incidence of bacteria containing R factors in sewage appears to be increasing with time, and their removal from sewage before discharge to the receiving environment is desirable. Consideration of data on bacteria with R factors should be made in future water quality deliberations and in discharge regulations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S. Origin of transferable drug-resistance factors in the enterobacteriaceae. Br Med J. 1965 Nov 27;2(5473):1289–1291. doi: 10.1136/bmj.2.5473.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson E. S. The ecology of transferable drug resistance in the enterobacteria. Annu Rev Microbiol. 1968;22:131–180. doi: 10.1146/annurev.mi.22.100168.001023. [DOI] [PubMed] [Google Scholar]
  3. Baine W. B., Farmer J. J., 3rd, Gangarosa E. J., Hermann G. T., Thornsberry C., Rice P. A. Typhoid fever in the United States associated with the 1972-1973 epidemic in Mexico. J Infect Dis. 1977 Apr;135(4):649–653. doi: 10.1093/infdis/135.4.649. [DOI] [PubMed] [Google Scholar]
  4. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  5. Bell J. B., Macrae W. R., Elliott G. E. Incidence of R factors in coliform, fecal coliform, and Salmonella populations of the Red River in Canada. Appl Environ Microbiol. 1980 Sep;40(3):486–491. doi: 10.1128/aem.40.3.486-491.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bell R. B. Antibiotic resistance patterns of fecal coliforms isolated from domestic sewage before and after treatment in an aerobic lagoon. Can J Microbiol. 1978 Jul;24(7):886–888. doi: 10.1139/m78-147. [DOI] [PubMed] [Google Scholar]
  7. Feary T. W., Sturtevant A. B., Jr, Lankford J. Antibiotic-resistant coliforms in fresh and salt water. Arch Environ Health. 1972 Sep;25(3):215–220. doi: 10.1080/00039896.1972.10666163. [DOI] [PubMed] [Google Scholar]
  8. Gangarosa E. J., Bennett J. V., Wyatt C., Pierce P. E., Olarte J., Hernandes P. M., Vázquez V., Bessudo D. An epidemic-associated episome? J Infect Dis. 1972 Aug;126(2):215–218. doi: 10.1093/infdis/126.2.215. [DOI] [PubMed] [Google Scholar]
  9. Smith P. R., Farrell E., Dunican K. Survival of R+ Escherichia coli in sea water. Appl Microbiol. 1974 May;27(5):983–984. doi: 10.1128/am.27.5.983-984.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sturtevant A. B., Cassell G. H., Feary T. W. Incidence of infectious drug resistance among fecal coliforms isolated from raw sewage. Appl Microbiol. 1971 Mar;21(3):487–491. doi: 10.1128/am.21.3.487-491.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sturtevant A. B., Jr, Feary T. W. Incidence of infectious drug resistance among lactose-fermenting bacteria isolated from raw and treated sewage. Appl Microbiol. 1969 Nov;18(5):918–924. doi: 10.1128/am.18.5.918-924.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomas K. L. A simple method of demonstrating transferable drug resistance. Med Lab Technol. 1975 Oct;32(4):313–315. [PubMed] [Google Scholar]

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