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. 1981 Sep;42(3):533–540. doi: 10.1128/aem.42.3.533-540.1981

Relationship of Cell Envelope Stability to Substrate Capture in a Marine Psychrophilic Bacterium †

Gill G Geesey 1, Richard Y Morita 2
PMCID: PMC244049  PMID: 16345849

Abstract

Cells of a psychrophilic marine bacterium were found to take up a variety of amino acids from seawater. Some of the amino acids that were taken up were released when the cells were exposed to a hypotonic salt solution. The proportion that was released varied according to the amino acid. A pool of the amino acid arginine that was formed during very short periods of exposure of cells to the exogenously supplied amino acid was particularly sensitive to reductions in salinity. In general, exposure to hypotonic salt solutions also resulted in reduced amino acid uptake by the cells. Complete removal of seawater salts (SE treatment) produced obvious structural alterations in the cell envelope, resulting in an even greater reduction in amino acid uptake. Under these conditions, amino acid-binding components were released by the cells. Differential centrifugation and fluorescent antibody studies indicated that arginine-binding components are located on or near the surface of intact cells. The data suggest that substrate receptors were sensitive to reductions in seawater salt concentrations and that lesions at this level affected the organism's substrate uptake and retention capabilities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baross J. A., Hanus F. J., Morita R. Y. Survival of human enteric and other sewage microorganisms under simulated deep-sea conditions. Appl Microbiol. 1975 Aug;30(2):309–318. doi: 10.1128/am.30.2.309-318.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Costerton J. W., Forsberg C., Matula T. I., Buckmire F. L., MacLeod R. A. Nutrition and metabolism of marine bacteria. XVI. Formation of protoplasts, spheroplasts, and related forms from a gram-negative marine bacterium. J Bacteriol. 1967 Nov;94(5):1764–1777. doi: 10.1128/jb.94.5.1764-1777.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Costerton J. W. Relationship of a wall-associated enzyme with specific layers of the cell wall of a gram-negative bacterium. J Bacteriol. 1973 Jun;114(3):1281–1293. doi: 10.1128/jb.114.3.1281-1293.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dills S. S., Apperson A., Schmidt M. R., Saier M. H., Jr Carbohydrate transport in bacteria. Microbiol Rev. 1980 Sep;44(3):385–418. doi: 10.1128/mr.44.3.385-418.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forsberg C. W., Costerton J. W., Macleod R. A. Separation and localization of cell wall layers of a gram-negative bacterium. J Bacteriol. 1970 Dec;104(3):1338–1353. doi: 10.1128/jb.104.3.1338-1353.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Furlong C. E., Morris R. G., Kandrach M., Rosen B. P. A multichamber equilibrium dialysis apparatus. Anal Biochem. 1972 Jun;47(2):514–526. doi: 10.1016/0003-2697(72)90146-7. [DOI] [PubMed] [Google Scholar]
  7. Geesey G. G., Costerton J. W. Microbiology of a northern river: bacterial distribution and relationship to suspended sediment and organic carbon. Can J Microbiol. 1979 Sep;25(9):1058–1062. doi: 10.1139/m79-162. [DOI] [PubMed] [Google Scholar]
  8. Geesey G. G., Morita R. Y. Capture of arginine at low concentrations by a marine psychrophilic bacterium. Appl Environ Microbiol. 1979 Dec;38(6):1092–1097. doi: 10.1128/aem.38.6.1092-1097.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Geesey G. G., Morita R. Y. Some physiological effects of near-maximum growth temperatures on an obligately psychrophilic marine bacterium. Can J Microbiol. 1975 Jun;21(6):811–818. doi: 10.1139/m75-120. [DOI] [PubMed] [Google Scholar]
  10. Griffiths R. P., Baross J. A., Hanus F. J., Morita R. Y. Some physical and chemical parameters affecting the formation and retention of glutamate pools in a marine psychrophilic bacterium. Z Allg Mikrobiol. 1974;14(5):359–369. doi: 10.1002/jobm.3630140502. [DOI] [PubMed] [Google Scholar]
  11. HAHN J. J., COLE R. M. STREPTOCOCCAL M ANTIGEN LOCATION AND SYNTHESIS, STUDIED BY IMMUNOFLUORESCENCE. J Exp Med. 1963 Nov 1;118:659–666. doi: 10.1084/jem.118.5.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kustu S. G., Ames G. F. The histidine-binding protein J, a histidine transport component, has two different functional sites. J Biol Chem. 1974 Nov 10;249(21):6976–6983. [PubMed] [Google Scholar]
  13. LOEFFLER H., HENLE G., HENLE W. Attempts to influence the incomplete reproductive cycle of influenza virus in HeLa cells by antibodies. J Immunol. 1962 Jun;88:763–776. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Lever J. E. Purification and properties of a component of histidine transport in Salmonella typhimurium. The histidine-binding protein J. J Biol Chem. 1972 Jul 10;247(13):4317–4326. [PubMed] [Google Scholar]
  16. Novitsky J. A., Morita R. Y. Survival of a psychrophilic marine Vibrio under long-term nutrient starvation. Appl Environ Microbiol. 1977 Mar;33(3):635–641. doi: 10.1128/aem.33.3.635-641.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwarz J. R., Colwell R. R. Heterotrophic activity of deep-sea sediment bacteria. Appl Microbiol. 1975 Oct;30(4):639–649. doi: 10.1128/am.30.4.639-649.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stevenson J. The specific requirement for sodium chloride for the active uptake of L-glutamate by Halobacterium salinarium. Biochem J. 1966 May;99(2):257–260. doi: 10.1042/bj0990257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ward B. B., Perry M. J. Immunofluorescent Assay for the Marine Ammonium-Oxidizing Bacterium Nitrosococcus oceanus. Appl Environ Microbiol. 1980 Apr;39(4):913–918. doi: 10.1128/aem.39.4.913-918.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson O. H., Holden J. T. Stimulation of arginine transport in osmotically shocked Escherichia coli W cells by purified arginine-binding protein fractions. J Biol Chem. 1969 May 25;244(10):2743–2749. [PubMed] [Google Scholar]

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