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. Author manuscript; available in PMC: 2009 Jun 5.
Published in final edited form as: Virology. 2008 Apr 1;375(2):480–491. doi: 10.1016/j.virol.2008.02.030

Figure 3. CVB3/5NTRMitoTD23 replicates without cytopathic effect but restoration of 5’ terminal sequence to HEV-B consensus results in cytopathic replication.

Figure 3

(A) HeLa cell monolayers were transfected with T7 RNA polymerase transcripts of pCMVT7r5NTRMitoTD23 (CVB3/5NTRMitoTD23), pCMVT7r5Mito (CVB3/5NTRMito), pCVB3−28 (CVB3/28) or mock transfected, control. Monolayers were fixed at 72 hours and stained with crystal violet. (B) cDNA from RNA of 72 hour cultures of transfected HeLa monolayers was amplified with primer E1 and primers S1ENTB (lane 1), S2ENTB (lane 2), S3ENTB (lane 3), S4ENTB (lane 4) and electrophoresed in 1.5% agarose. Lane M, Hi-Lo DNA Marker (Minnesota Molecular, Minneapolis, MN); lane -, PCR with S4ENTB and E1 without template cDNA. Arrow indicates 750 bp.