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. 1981 Oct;42(4):615–618. doi: 10.1128/aem.42.4.615-618.1981

Improved Isolation of Salmonellae from Naturally Contaminated Meat Products by Using Rappaport-Vassiliadis Enrichment Broth

Peter Vassiliadis 1, Victoria Kalapothaki 2, Dimitrios Trichopoulos 2, Christofili Mavrommatti 1, Charles Serie 1
PMCID: PMC244071  PMID: 7337465

Abstract

A total of 454 specimens of meat products were examined for salmonellae by using five procedures of enrichment. The use of a selective motility medium, inoculated from enrichment in Muller-Kauffmann broth, resulted in an increase in the number of positive specimens. However, simple enrichment in Rappaport-Vassiliadis broth, after preenrichment, was more sensitive and specific for recovering salmonellae than the selective motility medium-Muller-Kauffmann broth method.

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Selected References

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  1. Harper J., Shortridge K. F. A selective motility medium for routine isolation of Salmonella. J Hyg (Lond) 1969 Jun;67(2):181–186. doi: 10.1017/s0022172400041589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harvey R. W., Mahabir D. E., Price T. H. A method of secondary enrichment for salmonellas independent of selectively toxic chemicals. J Hyg (Lond) 1966 Sep;64(3):361–366. doi: 10.1017/s002217240004064x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harvey R. W., Price T. H., Xirouchaki E. Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. J Hyg (Lond) 1979 Dec;83(3):451–460. doi: 10.1017/s0022172400026292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KONFORTI N., NAVON B., RAPPAPORT F. A new enrichment medium for certain Salmonellae. J Clin Pathol. 1956 Aug;9(3):261–266. doi: 10.1136/jcp.9.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Smeltzer T. I., Duncalfe F. Secondary selective enrichment of salmonellae from naturally contaminated specimens by using a selective motility system. Appl Environ Microbiol. 1979 Apr;37(4):725–728. doi: 10.1128/aem.37.4.725-728.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Thomason B. M., Dodd D. J. Enrichment procedures for isolating salmonellae from raw meat and poultry. Appl Environ Microbiol. 1978 Oct;36(4):627–628. doi: 10.1128/aem.36.4.627-628.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Vassiliadis P., Patéraki E., Papaïconomou N., Papadakis J. A., Trichopoulos D. Noueau procédé d'enrichissement de Salmonella. Ann Microbiol (Paris) 1976 Aug-Sep;127B(2):195–200. [PubMed] [Google Scholar]
  8. Vassiliadis P., Trichopoulos D., Kalandidi A., Xirouchaki E. Isolation of Salmonellae from sewage with a new procedure of enrichment. J Appl Bacteriol. 1978 Apr;44(2):233–239. doi: 10.1111/j.1365-2672.1978.tb00795.x. [DOI] [PubMed] [Google Scholar]
  9. Vassiliadis P., Trichopoulos D., Kalapothaki V., Sérié C. Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium. J Hyg (Lond) 1981 Aug;87(1):35–41. doi: 10.1017/s0022172400069217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vassiliadis P., Trichopoulos D., Papadakis J., Kalapothaki V., Serie C. Brilliant green deoxycholate agar as an improved selective medium for the isolation of Salmonella. Ann Soc Belg Med Trop. 1979 Jun;59(2):117–120. [PubMed] [Google Scholar]
  11. Vassiliadis P., Trichopoulos D., Papadakis J., Kalapothaki V., Zavitsanos X., Serie C. Salmonella isolation with Rappaport's enrichment medium of different compositions. Zentralbl Bakteriol Mikrobiol Hyg B. 1981;173(5):382–389. [PubMed] [Google Scholar]
  12. Vassiliadis P., Trichopoulos D., Pateraki E., Papaiconomou N. Isolation of salmonella from minced meat by the use of a new procedure of enrichment. Zentralbl Bakteriol Orig B. 1978 Jan;166(1):81–86. [PubMed] [Google Scholar]

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