Abstract
Problems associated with overgrowth by spreading molds are not addressed by currently recommended fungal enumeration media. Twenty-two fungi, including 12 mold and 3 yeast genera, were evaluated for the effects of dichloran (2,6-dichloro-4-nitroaniline), previously identified as a mold-spreading inhibitor, on colony diameter and enumeration. On malt agar or antibiotic-potato-dextrose agar (APDA), colony diameters were effectively reduced when dichloran was added. Colony diameters decreased as the dichloran concentration increased. Counts obtained with mixed mold spore suspensions were lower on APDA supplemented with 25 μg of dichloran per ml than on APDA and were higher than APDA with the addition of 5 μg of dichloran per ml (APDA-D-5). Overall counts of mixed and individual mold spore and yeast suspensions were higher in APDA-D-5 than in APDA. The additional advantages of APDA-D-5 may be useful in routine enumeration of fungi.
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- Bell D. K., Crawford J. L. A botran-amended medium for isolating Aspergillus flavus from peanuts and soil. Phytopathology. 1967 Sep;57(9):939–941. [PubMed] [Google Scholar]
- Hocking A. D., Pitt J. I. Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods. Appl Environ Microbiol. 1980 Mar;39(3):488–492. doi: 10.1128/aem.39.3.488-492.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jarvis B. Comparison of an improved rose bengal-chlortetracycline agar with other media for the selective isolation and enumeration of moulds and yeasts in foods. J Appl Bacteriol. 1973 Dec;36(4):723–727. doi: 10.1111/j.1365-2672.1973.tb04157.x. [DOI] [PubMed] [Google Scholar]
- King A. D., Jr, Hocking A. D., Pitt J. I. Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Appl Environ Microbiol. 1979 May;37(5):959–964. doi: 10.1128/aem.37.5.959-964.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mislivec P. B. The effect of Botran on fascicle production by species of Penicillium. Mycologia. 1975 Jan-Feb;67(1):194–198. [PubMed] [Google Scholar]
- Mossel D. A., Vega C. L., Put H. M. Further studies on the suitability of various media containing antibacterial antibiotics for the enumeration of moulds in food and food environments. J Appl Bacteriol. 1975 Aug;39(1):15–22. doi: 10.1111/j.1365-2672.1975.tb00540.x. [DOI] [PubMed] [Google Scholar]
- Nelson F. E. Plating medium pH as a factor in apparent survival of sublethally stressed yeasts. Appl Microbiol. 1972 Aug;24(2):236–239. doi: 10.1128/am.24.2.236-239.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ottow J. C., Glathe H. Rose bengal-malt extract-agar, a simple medium for the simultaneous isolation and enumeration of fungi and actinomycetes from soil. Appl Microbiol. 1968 Jan;16(1):170–171. doi: 10.1128/am.16.1.170-171.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]