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. 1981 Oct;42(4):661–666. doi: 10.1128/aem.42.4.661-666.1981

Isolation of virulent Yersinia enterocolitica from porcine tongues.

M P Doyle, M B Hugdahl, S L Taylor
PMCID: PMC244079  PMID: 7337466

Abstract

Thirty-one tongues from apparently normal, freshly slaughtered pigs were assayed for the presence of Yersinia enterocolitica by different enrichment and postenrichment techniques. Sixteen different isolates were recovered, including six of serotype O:8, four of serotype O:6,30, two of serotype O:3 phage type IXb, and one each of serotypes O:13,7, O:18, and O:46. One isolate was not typable. Cold enrichment in phosphate-buffered saline followed by treatment with dilute KOH or subsequent enrichment in modified Rappaport broth recovered 12 and 7 isolates, respectively. Four of the same isolates were recovered from the same tongues by both procedures. Cold enrichment without a selective postenrichment treatment recovered two isolates. Direct enrichment in modified Rappaport broth or modified selenite broth was ineffective in recovering yersiniae, as no isolates were obtained by either method. All of the serotype O:8 isolates were virulent to mice, causing the death of adults after oral challenge. This is the first report that associates Y. enterocolitica serotype O:8 with a natural reservoir.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aulisio C. C., Mehlman I. J., Sanders A. C. Alkali method for rapid recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods. Appl Environ Microbiol. 1980 Jan;39(1):135–140. doi: 10.1128/aem.39.1.135-140.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bissett M. L. Yersinia enterocolitica isolates from humans in California, 1968-1975. J Clin Microbiol. 1976 Aug;4(2):137–144. doi: 10.1128/jcm.4.2.137-144.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Black R. E., Jackson R. J., Tsai T., Medvesky M., Shayegani M., Feeley J. C., MacLeod K. I., Wakelee A. M. Epidemic Yersinia enterocolitica infection due to contaminated chocolate milk. N Engl J Med. 1978 Jan 12;298(2):76–79. doi: 10.1056/NEJM197801122980204. [DOI] [PubMed] [Google Scholar]
  4. Bottone E. J. Yersinia enterocolitica: a panoramic view of a charismatic microorganism. CRC Crit Rev Microbiol. 1977;5(2):211–241. doi: 10.3109/10408417709102312. [DOI] [PubMed] [Google Scholar]
  5. Carter P. B., Varga C. F., Keet E. E. New strain of Yersinia enterocolitica pathogenic for rodents. Appl Microbiol. 1973 Dec;26(6):1016–1018. doi: 10.1128/am.26.6.1016-1018.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Christensen S. G. Yersinia enterocolitica in Danish pigs. J Appl Bacteriol. 1980 Jun;48(3):377–382. doi: 10.1111/j.1365-2672.1980.tb01025.x. [DOI] [PubMed] [Google Scholar]
  7. Eiss J. Selective culturing of Yersinia enterocolitica at a low temperature. Scand J Infect Dis. 1975;7(4):249–251. doi: 10.3109/inf.1975.7.issue-4.05. [DOI] [PubMed] [Google Scholar]
  8. Falcao D. P., Ewing W. H., Dowell V. R., Jr Cultural characteristics of Yersinia enterocolitica and Yersinia pseudotuberculosis on differential media. Contrib Microbiol Immunol. 1979;5:88–94. [PubMed] [Google Scholar]
  9. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greenwood J. R., Flanigan S. M., Pickett M. J., Martin W. J. Clinical isolation of Yersinia enterocolitica: cold temperature enrichment. J Clin Microbiol. 1975 Dec;2(6):559–560. doi: 10.1128/jcm.2.6.559-560.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hurvell B., Glatthard V., Thal E. Isolation of Yersinia enterocolitica from swine at an abattoir in Sweden. Contrib Microbiol Immunol. 1979;5:243–248. [PubMed] [Google Scholar]
  12. Laird W. J., Cavanaugh D. C. Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980 Apr;11(4):430–432. doi: 10.1128/jcm.11.4.430-432.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee W. H., Harris M. E., McClain D., Smith R. E., Johnston R. W. Two modified selenite media for the recovery of Yersinia enterocolitica from meats. Appl Environ Microbiol. 1980 Jan;39(1):205–209. doi: 10.1128/aem.39.1.205-209.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee W. H. Testing for the recovery of Yersinia enterocolitica in foods and their ability to invade HeLa cells. Contrib Microbiol Immunol. 1979;5:228–233. [PubMed] [Google Scholar]
  15. Mehlman I. J., Aulisio C. C., Sanders A. C. Microbiological methods. Problems in the recovery and identification of Yersinia from food. J Assoc Off Anal Chem. 1978 Jul;61(4):761–771. [PubMed] [Google Scholar]
  16. Mosimann J., Bonifas V., Brunner S., Jung M. Contribution au sérodiagnostic des infections humaines à Yersinia. Résultats préliminaires. Schweiz Med Wochenschr. 1980 Nov 1;110(44):1605–1610. [PubMed] [Google Scholar]
  17. Pai C. H., Sorger S., Lafleur L., Lackman L., Marks M. I. Efficacy of cold enrichment techniques for recovery of Yersinia enterocolitica from human stools. J Clin Microbiol. 1979 Jun;9(6):712–715. doi: 10.1128/jcm.9.6.712-715.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pedersen K. B. Isolation of Yersinia enterocolitica from Danish swine and dogs. Acta Pathol Microbiol Scand B. 1976 Oct;84B(5):317–318. doi: 10.1111/j.1699-0463.1976.tb01945.x. [DOI] [PubMed] [Google Scholar]
  19. Pedersen K. B. Occurrence of Yersinia enterocolitica in the throat of swine. Contrib Microbiol Immunol. 1979;5:253–256. [PubMed] [Google Scholar]
  20. Pedersen K. B., Winblad S. Studies on Yersinia enterocolitica isolated from swine and dogs. Acta Pathol Microbiol Scand B. 1979 Apr;87B(2):137–140. doi: 10.1111/j.1699-0463.1979.tb02416.x. [DOI] [PubMed] [Google Scholar]
  21. Schiemann D. A. Enrichment methods for recovery of Yersinia enterocolitica from foods and raw milk. Contrib Microbiol Immunol. 1979;5:212–227. [PubMed] [Google Scholar]
  22. Toma S., Deidrick V. R. Isolation of Yersinia enterocolitica from swine. J Clin Microbiol. 1975 Dec;2(6):478–481. doi: 10.1128/jcm.2.6.478-481.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Toma S., Lafleur L., Deidrick V. R. Canadian experience with Yersinia enterocolitica (1966--1977). Contrib Microbiol Immunol. 1979;5:144–149. [PubMed] [Google Scholar]
  24. Tsubokura M., Otsuki K., Itagaki K. Studies on Yersinia enterocolitica. I. Isolation of Y. enterocolitica from swine. Nihon Juigaku Zasshi. 1973 Oct;35(5):419–424. doi: 10.1292/jvms1939.35.419. [DOI] [PubMed] [Google Scholar]
  25. Van Noyen R., Vandepitte J. L'isolement de Yersinia enterocolitica par une technique usuelle de coproculture. Ann Inst Pasteur (Paris) 1968 Apr;114(4):463–467. [PubMed] [Google Scholar]
  26. Van Noyen R., Vandepitte J., Wauters G. Nonvalue of cold enrichment of stools for isolation of Yersinia enterocolitica serotypes 3 and 9 from patients. J Clin Microbiol. 1980 Feb;11(2):127–131. doi: 10.1128/jcm.11.2.127-131.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wauters G. Carriage of Yersinia enterocolitica serotype 3 by pigs as a source of human infection. Contrib Microbiol Immunol. 1979;5:249–252. [PubMed] [Google Scholar]
  28. Weissfeld A. S., Sonnenwirth A. C. Yersinia enterocolitica in adults with gastrointestinal disturbances: need for cold enrichment. J Clin Microbiol. 1980 Feb;11(2):196–197. doi: 10.1128/jcm.11.2.196-197.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zen-Yoji H., Sakai S., Maruyama T., Yanagawa Y. Isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis from swine, cattle and rats at an abattoir. Jpn J Microbiol. 1974 Jan;18(1):103–105. doi: 10.1111/j.1348-0421.1974.tb00753.x. [DOI] [PubMed] [Google Scholar]
  30. van Pee W., Stragier J. Evaluation of some cold enrichment and isolation media for the recovery of Yersinia enterocolitica. Antonie Van Leeuwenhoek. 1979;45(3):465–477. doi: 10.1007/BF00443284. [DOI] [PubMed] [Google Scholar]

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