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. 1981 Oct;42(4):704–707. doi: 10.1128/aem.42.4.704-707.1981

Modified enrichment-serology procedure for detection of salmonellae in soy products.

T E Surdy, S O Haas
PMCID: PMC244087  PMID: 7199894

Abstract

A modified enrichment-serology (MES) procedure was used to reduce the time necessary for salmonella analysis. Naturally contaminated samples of soy products were preenriched in 1% proteose peptone for 6 h at 37 degrees C followed by inoculation into tetrathionate broth for 18 h at 37 degrees C. Two drops of the tetrathionate sample were inoculated into M broth. After incubation at 37 degrees C for 6 h, 0.85 ml of the mixture was formolized, and 0.1 ml of polyvalent H antiserum was added. After incubation in water bath at 50 degrees C for 1 h, the appearance of a typical floccular flagellar precipitate was observed in tubes positive for salmonellae. Over 3,000 samples were subjected to standard biochemical and serological procedures, and the results were compared with those of the MES method with a 96.7% correlation. Eleven of the samples (0.3%) were false-negative with the MES procedure, and 3% were false-negative with the U.S. Food and Drug Administration Bacteriological Analytical Manual procedure. The 3% negative samples by this latter procedure were subsequently found to be positive by the MES procedure. The MES procedure reduced the time required for salmonella analysis from 4 days to 32 h.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barkate J. A. Screening of feed components for Salmonella with polyvalent H agglutination. Appl Microbiol. 1968 Dec;16(12):1872–1874. doi: 10.1128/am.16.12.1872-1874.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boothroyd M., Baird-Parker A. C. The use of enrichment serology for Salmonella detection in human foods and animal feeds. J Appl Bacteriol. 1973 Mar;36(1):165–172. doi: 10.1111/j.1365-2672.1973.tb04084.x. [DOI] [PubMed] [Google Scholar]
  3. Carlson V. L., Snoeyenbos G. H. Comparative efficacies of selenite and tetrathionate enrichment broths for the isolation of Salmonella serotypes. Am J Vet Res. 1974 May;35(5):711–718. [PubMed] [Google Scholar]
  4. Edel W., Kampelmacher E. H. Comparative studies on the isolation of "sublethally injured" salmonellae in nine European laboratories. Bull World Health Organ. 1973;48(2):167–174. [PMC free article] [PubMed] [Google Scholar]
  5. Fantasia L. D., Sperber W. H., Deibel R. H. Comparison of two procedures for detection of Salmonella in food, feed, and pharmaceutical products. Appl Microbiol. 1969 Apr;17(4):540–541. doi: 10.1128/am.17.4.540-541.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gabis D. A., Silliker J. H. ICMSF methods studies. IX. The influence of selective enrichment broths, differential planting media, and incubation temperatues on the detection of Salmonella in dried foods and feed ingredients. Can J Microbiol. 1977 Sep;23(9):1225–1231. doi: 10.1139/m77-183. [DOI] [PubMed] [Google Scholar]
  7. Gabis D. A., Silliker J. H. ICMSF methods studies. VI. The influence of selective enrichment media and incubation temperatures on the detection of Salmonella in dried foods and feeds. Can J Microbiol. 1974 Nov;20(11):1509–1511. doi: 10.1139/m74-235. [DOI] [PubMed] [Google Scholar]
  8. Kafel S., Bryan F. L. Effects of enrichment media and incubation conditions on isolating salmonellae from ground-meat filtrate. Appl Environ Microbiol. 1977 Sep;34(3):285–291. doi: 10.1128/aem.34.3.285-291.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mohr H. K., Trenk H. L., Yeterian M. Comparison of fluorescent-antibody methods and enrichment serology for the detection of Salmonella. Appl Microbiol. 1974 Feb;27(2):324–328. doi: 10.1128/am.27.2.324-328.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mossel D. A., Ratto M. A. Rapid detection of sublethally impaired cells of Enterobacteriaceae in dried foods. Appl Microbiol. 1970 Aug;20(2):273–275. doi: 10.1128/am.20.2.273-275.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nabbut N. H. Elevated temperature technique for the isolation of salmonellas from sewage and human faeces. J Hyg (Lond) 1973 Mar;71(1):49–54. doi: 10.1017/s0022172400046209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phirke P. M. Application of the rapid lysine decarboxylase test for early isolation and detection of salmonellae in sewage and other wastewaters. Appl Environ Microbiol. 1977 Oct;34(4):453–455. doi: 10.1128/aem.34.4.453-455.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sveum W. H., Hartman P. A. Timed-release capsule method for the detection of salmonellae in foods and feeds. Appl Environ Microbiol. 1977 Mar;33(3):630–634. doi: 10.1128/aem.33.3.630-634.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thomason B. M., Dodd D. J., Cherry W. B. Increased recovery of salmonellae from environmental samples enriched with buffered peptone water. Appl Environ Microbiol. 1977 Sep;34(3):270–273. doi: 10.1128/aem.34.3.270-273.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomason B. M., Dodd D. J. Enrichment procedures for isolating salmonellae from raw meat and poultry. Appl Environ Microbiol. 1978 Oct;36(4):627–628. doi: 10.1128/aem.36.4.627-628.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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