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. 1981 Oct;42(4):708–719. doi: 10.1128/aem.42.4.708-719.1981

Dissolved Hydrocarbons and Related Microflora in a Fjordal Seaport: Sources, Sinks, Concentrations, and Kinetics

D K Button 1, Betsy R Robertson 1, Kathleen S Craig 1
PMCID: PMC244088  PMID: 16345870

Abstract

The continuous addition of toluene as a solute of treated ballast water from oil tankers into a well-defined estuary facilitated the study of the dynamics of dissolved hydrocarbon metabolism in seawater. Most rates of toluene oxidation were in the range of 1 to 30 pg/liter per h at 0.5 μg of toluene per liter. Near the ballast water injection point, a layer of warm ballast water, rich in bacteria, that was trapped below the less-dense fresh surface water was located. Toluene residence times were approximately 2 weeks in this layer, 2 years elsewhere in Port Valdez, and 2 decades in the surface water of a more oceanic receiving estuary adjacent. Mixing was adequate for a steady-state treatment which showed that 98% of the toluene was flushed from Port Valdez before metabolism and gave a steady-state concentration of 0.18 μg/liter. Total bacterial biomass from direct counts and organism size data was usually near 0.1 mg/liter, but ranged up to 0.8 mg/liter in the bacteria-rich layer. The origin of bacteria in this layer was traced to growth in oil tanker ballast during shipments. The biomass of toluene oxidizers in water samples was estimated from the average affinity of pure-culture isolates for toluene (28 liters per g of cells per h) and observed toluene oxidation kinetics. Values ranged from nearly all of the total bacterial biomass within the bacteria-rich layer down to 0.2% at points far removed. Because the population of toluene oxidizers was large with respect to the amount of toluene consumed and because water from a nearby nonpolluted estuary was equally active in facilitating toluene metabolism, we searched for an additional hydrocarbon source. It was found that terpenes could be washed from spruce trees by simulated rainfall, which suggested that riparian conifers provide an additional and significant hydrocarbon source to seawater.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atlas R. M. Stimulated petroleum biodegradation. CRC Crit Rev Microbiol. 1977 Sep;5(4):371–386. doi: 10.3109/10408417709102810. [DOI] [PubMed] [Google Scholar]
  2. Bowden W. B. Comparison of two direct-count techniques for enumerating aquatic bacteria. Appl Environ Microbiol. 1977 May;33(5):1229–1232. doi: 10.1128/aem.33.5.1229-1232.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Button D. K., Schell D. M., Robertson B. R. Sensitive and accurate methodology for measuring the kinetics of concentration-dependent hydrocarbon metabolism rates in seawater by microbial communities. Appl Environ Microbiol. 1981 Apr;41(4):936–941. doi: 10.1128/aem.41.4.936-941.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. I. General properties as studied in a galactokinaseless mutant. J Biol Chem. 1960 Jun;235:1580–1585. [PubMed] [Google Scholar]
  5. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Law A. T., Button D. K. Multiple-carbon-source-limited growth kinetics of a marine coryneform bacterium. J Bacteriol. 1977 Jan;129(1):115–123. doi: 10.1128/jb.129.1.115-123.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Patel R. N., Hou C. T., Laskin A. I., Felix A., Derelanko P. Microbial oxidation of gaseous hydrocarbons. II. Hydroxylation of alkanes and epoxidation of alkenes by cell-free particulate fractions of methane-utilizing bacteria. J Bacteriol. 1979 Aug;139(2):675–679. doi: 10.1128/jb.139.2.675-679.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Robertson B. R., Button D. K. Phosphate-limited continuous culture of Rhodotorula rubra: kinetics of transport, leakage, and growth. J Bacteriol. 1979 Jun;138(3):884–895. doi: 10.1128/jb.138.3.884-895.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roubal G., Atlas R. M. Distribution of hydrocarbon-utilizing microorganisms and hydrocarbon biodegradation potentials in Alaskan continental shelf areas. Appl Environ Microbiol. 1978 May;35(5):897–905. doi: 10.1128/aem.35.5.897-905.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ward D. M., Brock T. D. Environmental factors influencing the rate of hydrocarbon oxidation in temperate lakes. Appl Environ Microbiol. 1976 May;31(5):764–772. doi: 10.1128/aem.31.5.764-772.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Yoshida F., Yamane T., Yagi H. Mechanism of uptake of liquid hydrocarbons by microorganisms. Biotechnol Bioeng. 1971 Mar;13(2):215–228. doi: 10.1002/bit.260130205. [DOI] [PubMed] [Google Scholar]

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