Abstract
Scrapings of decomposing Cladophora sp. mats (periphyton) covering stream bed rocks produced N2O when incubated under N2 plus 15% C2H2. Denitrification (N2O formation) was enhanced by NO3− and was inhibited by autoclaving, Hg2+, and O2. No N2O was formed in the absence of C2H2 (air or N2 atmosphere). Chloramphenicol did not block N2O formation, indicating that the enzymes were constitutive. In field experiments, incubation of periphyton scrapings in the light inhibited denitrification because of algal photosynthetic O2 production. The diurnal periphyton-associated denitrification rate was estimated to be 45.8 μmol of N2O·m−2·day−1, as determined by averaging light, aerobic plus dark, and anaerobic rates over a 24-h period.
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