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. 1981 Nov;42(5):773–778. doi: 10.1128/aem.42.5.773-778.1981

Production of l-Phenylalanine from trans-Cinnamic Acid with Rhodotorula glutinis Containing l-Phenylalanine Ammonia-Lyase Activity

Shigeki Yamada 1, Koichi Nabe 1, Nobuhiko Izuo 1, Katsuhiko Nakamichi 1, Ichiro Chibata 1
PMCID: PMC244106  PMID: 16345879

Abstract

An enzymatic method using l-phenylalanine ammonia-lyase (EC 4.3.1.5) for the rapid conversion of trans-cinnamic acid to l-phenylalanine has been investigated. With Rhodotorula glutinis, enzyme activity as high as 0.3 U/ml of culture broth was obtained. The enzyme activity was kept stable for a relatively long time during cultivation by the addition of l-isoleucine. Optimization of the parameters of the conversion reaction resulted in accumulation of 18 mg of l-phenylalanine per ml of reaction mixture. The conversion yield from trans-cinnamic acid was about 70%. The method may provide a rapid and practical way to produce l-phenylalanine useful as an essential amino acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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