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. 1981 Nov;42(5):897–903. doi: 10.1128/aem.42.5.897-903.1981

Transduction of Lactose Metabolism by Streptococcus cremoris C3 Temperate Phage †

René J Snook 1, Larry L McKay 1, Gilbert G Ahlstrand 2
PMCID: PMC244124  PMID: 16345892

Abstract

Temperate phage was induced from Streptococcus cremoris C3 and morphologically characterized by high-resolution electron micrographic techniques. Interspecies genetic transfer of lactose-fermenting ability by the temperate phage was demonstrated, using two lactose-negative (Lac−) S. lactis strains as recipients. Plasmid transfer was confirmed by agarose gel electrophoresis. Transductant plasmid profiles were of three types—those containing no visible plasmid deoxyribonucleic acid, those possessing a 23-megadalton (Mdal) plasmid, and those containing a 23-Mdal plasmid and a 30-Mdal plasmid. A Lac+ transductant could serve as a donor of the lac determinants during solid-surface matings. These results add to previously published reports of inter- and intraspecies genetic transfer in dairy starter cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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