Abstract
A group of fecal isolates identified as Eubacterium lentum elaborated 3 alpha-, 7 alpha-, and 12 alpha-dehydrogenases and also an epimerizing enzyme(s) for the 3 alpha-hydroxy group. The activities of the enzymes, however, were variably manifested according to the kind of bile acid substrate and the oxygen tension under which the reaction occurred.
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Selected References
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- Aries V., Hill M. J. Degradation of steroids by intestinal bacteria. II. Enzymes catalysing the oxidoreduction of the 3 alpha-, 7 alpha- and 12 alpha-hydroxyl groups in cholic acid, and the dehydroxylation of the 7-hydroxyl group. Biochim Biophys Acta. 1970 May 5;202(3):535–543. doi: 10.1016/0005-2760(70)90124-4. [DOI] [PubMed] [Google Scholar]
- Bokkenheuser V. D., Winter J., Dehazya P., Kelly W. G. Isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids. Appl Environ Microbiol. 1977 Nov;34(5):571–575. doi: 10.1128/aem.34.5.571-575.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris J. N., Hylemon P. B. Partial purification and characterization of NADP-dependent 12alpha-hydroxysteroid dehydrogenase from Clostridium leptum. Biochim Biophys Acta. 1978 Jan 27;528(1):148–157. doi: 10.1016/0005-2760(78)90060-7. [DOI] [PubMed] [Google Scholar]
- Hirano S., Masuda N., Oda H., Imamura T. Transformation of bile acids by mixed microbial cultures from human feces and bile acid transforming activities of isolated bacterial strains. Microbiol Immunol. 1981;25(3):271–282. doi: 10.1111/j.1348-0421.1981.tb00029.x. [DOI] [PubMed] [Google Scholar]
- Hirano S., Masuda N., Oda H., Mukai H. Transformation of bile acids by Clostridium perfringens. Appl Environ Microbiol. 1981 Sep;42(3):394–399. doi: 10.1128/aem.42.3.394-399.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirano S., Nakama R., Tamaki M., Masuda N., Oda H. Isolation and characterization of thirteen intestinal microorganisms capable of 7 alpha-dehydroxylating bile acids. Appl Environ Microbiol. 1981 Mar;41(3):737–745. doi: 10.1128/aem.41.3.737-745.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonald I. A., Jellett J. F., Mahony D. E., Holdeman L. V. Bile salt 3 alpha- and 12 alpha-hydroxysteroid dehydrogenases from Eubacterium lentum and related organisms. Appl Environ Microbiol. 1979 May;37(5):992–1000. doi: 10.1128/aem.37.5.992-1000.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonald I. A., Mahony D. E., Jellet J. F., Meier C. E. NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559. Biochim Biophys Acta. 1977 Dec 21;489(3):466–476. doi: 10.1016/0005-2760(77)90167-9. [DOI] [PubMed] [Google Scholar]
- Macdonald I. A., Forrest T. P., Costain G. A., Rao B. G. Identification of 7alpha-, 12alpha-dihydroxy-3-oxo cholanoic acid as the major degradation product from cholic by C. perfringens. J Steroid Biochem. 1978 Apr;9(4):353–358. doi: 10.1016/0022-4731(78)90630-1. [DOI] [PubMed] [Google Scholar]
- Midtvedt T., Norman A. Bile acid transformations by microbial strains belonging to genera found in intestinal contents. Acta Pathol Microbiol Scand. 1967;71(4):629–638. doi: 10.1111/j.1699-0463.1967.tb05183.x. [DOI] [PubMed] [Google Scholar]