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. 1981 Nov;42(5):921–924. doi: 10.1128/aem.42.5.921-924.1981

Positively charged filters for virus recovery from wastewater treatment plant effluents.

L T Chang, S R Farrah, G Bitton
PMCID: PMC244129  PMID: 6274257

Abstract

Positively charged Zeta Plus filters were used to concentrate enteroviruses from 19 liters of effluent from activated sludge units. Neither the addition of salts nor the acidification of the effluent was required for adsorption of viruses to the filters. Viruses adsorbed to the filters were eluted by treating the filters with a solution of 4 M urea buffered at pH 9 with 0.05 M lysine. Eluted viruses were concentrated into final volumes of 1 to 2 ml by using a two-step concentration procedure that employed inorganic and organic flocculation. Approximately 50% of the viruses added to effluents could be recovered in the final sample. The procedure was used to monitor effluents from activated sludge units at two wastewater treatment plants for the presence of enteroviruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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