Abstract
Spore components exuded into the medium during outgrowth of Bacillus cereus T enhanced the bacteriostatic effectiveness of S-nitrosomercaptoethanol, an inhibitor which prevents outgrowth at low concentrations and germination at higher concentrations. The enhancement effect was slight with respect to outgrowth, but dramatic with respect to germination, in that the inhibitory effectiveness of nitrosothiols toward germination inhibition was enhanced by as much as 33-fold when nitrosothiols was in the presence of the exuded spore component. Exudate activity was freely dialyzable and was not measurably affected by a broad-spectrum protease (proteinase K), by autoclaving at 121 degrees C, or by freezing and thawing. Sephadex G-25 chromatography of the exudate indicated that two active species were present, a major component with a molecular weight of less than 1,000 and a minor component with a molecular weight of more than 5,000.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hansen J. N., Levin R. A. Effect of some inhibitors derived from nitrite on marcomolecular synthesis in Bacillus cereus. Appl Microbiol. 1975 Nov;30(5):862–869. doi: 10.1128/am.30.5.862-869.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Incze K., Farkas J., Mihályi V., Zukál E. Antibacterial effect of cysteine-nitrosothiol and possible percursors thereof. Appl Microbiol. 1974 Jan;27(1):202–205. doi: 10.1128/am.27.1.202-205.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Leary V., Solberg M. Effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in Clostridium perfringens. Appl Environ Microbiol. 1976 Feb;31(2):208–212. doi: 10.1128/aem.31.2.208-212.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]